Akagi S, Kaneko H, Nakanishi Y, Takedomi T, Watanabe G, Taya K
Department of Animal Production, Kyushu National Agricultural Experiment Station, Kumamoto, Japan.
J Vet Med Sci. 1997 Dec;59(12):1129-35. doi: 10.1292/jvms.59.1129.
Dose effect of inhibin antiserum on ovarian response and hormonal profiles were investigated. On day 12 of the estrous cycle (day 0 = estrus), 14 of 19 cows were given a single i.v. injection of 25 ml (n = 4), 37.5 ml (n = 5) or 50 ml (n = 5) antiserum against inhibin produced in a castrated male goat. The other 5 animals were given 50 ml castrated male goat serum (control serum). The animals in each group received a single i.m. injection of 0.5 mg prostaglandin F2 alpha analogue (PG) 48 hr following the serum injection. The population of follicles and ovulation rate (estimated by the number of corpora lutea) were examined by ultrasonography. Administration of inhibin antiserum consistently resulted in a significant (p < 0.01) increase in plasma concentrations of follicle-stimulating hormone (FSH) in inhibin-neutralized groups, although the increased FSH levels were sustained longer in 50-ml group than in the 25- and 37.5- ml groups. Levels in the circulating inhibin antibody titer were positively correlated with dosage of inhibin antiserum. A large number of antral follicles (> or = 4 mm in diameter) developed similarly after hypersecretion of FSH in all neutralized groups, coupled with a rise in plasma estradiol levels, while the number of large follicles (> or = 10 mm in diameter) on estrus showed a dose-dependent increase. Multiple ovulation (2 to 4) was recorded in all animals after injection of 50 ml inhibin antiserum, however all cows in the 25-ml group experienced only one ovulation and injection of 37.5 ml resulted in a variable number of ovulations (1 to 5). These results demonstrated that administration of inhibin antiserum on day 12, followed by injection of PG, was able to induce hypersecretion of FSH and subsequently multiple ovulations. The number of large follicles on estrus day and ovulations were affected by dosage of inhibin antiserum and were correlated with persistence of increased FSH levels or circulating antibody levels.
研究了抑制素抗血清对卵巢反应和激素水平的剂量效应。在发情周期的第12天（第0天 = 发情期），19头奶牛中的14头静脉注射了25毫升（n = 4）、37.5毫升（n = 5）或50毫升（n = 5）针对去势雄山羊产生的抑制素的抗血清。另外5只动物注射了50毫升去势雄山羊血清（对照血清）。每组动物在注射血清48小时后肌肉注射0.5毫克前列腺素F2α类似物（PG）。通过超声检查卵泡数量和排卵率（根据黄体数量估算）。抑制素抗血清给药后，抑制素中和组的促卵泡激素（FSH）血浆浓度持续显著升高（p < 0.01），尽管50毫升组FSH水平升高的持续时间比25毫升和37.5毫升组更长。循环中抑制素抗体滴度水平与抑制素抗血清剂量呈正相关。在所有中和组中，FSH分泌过多后，大量窦状卵泡（直径≥4毫米）发育情况相似，同时血浆雌二醇水平升高，而发情期大卵泡（直径≥1毫米）数量呈剂量依赖性增加。注射50毫升抑制素抗血清后，所有动物均出现多次排卵（2至4次），然而25毫升组的所有奶牛仅排卵1次，注射37.5毫升则导致排卵次数不一（1至5次）。这些结果表明，在第12天注射抑制素抗血清，随后注射PG，能够诱导FSH分泌过多，进而导致多次排卵。发情期大卵泡数量和排卵次数受抑制素抗血清剂量影响，且与FSH水平升高的持续时间或循环抗体水平相关。
