Correa J R, Pace M M, Zavos P M
Department of Animal Sciences, University of Kentucky, Lexington, KY 40546, USA.
Theriogenology. 1997 Oct 1;48(5):721-31. doi: 10.1016/s0093-691x(97)00296-3.
Frozen semen specimens from 22 Holstein bulls representing a wide range of field fertility levels or nonreturn rates (NRR) were used in this study. Semen specimens were thawed at 37 degrees C for a minimum of 30 sec, followed by assessment via a routine semen analysis (RSA) and other sperm functional tests. The RSA was performed by assessing sperm count, motility and morphological characteristics. Other sperm functional tests were performed by assessing the acrosomal membrane integrity, sperm penetration into the cervical mucus and the sperm membrane functional integrity. Following assessment of sperm characteristics, the fertility data of the various bulls were compared to the RSA and the functional tests results. Bulls of high and low fertility were similar in terms of sperm count and progressive motility (P > 0.05). Other characteristics measured by the RSA and functional tests were significantly higher in high fertility bulls (P < 0.05). Correlation coefficients among the various sperm characteristics and fertility of bulls were highly significant (P < 0.01). The highest correlation coefficients between sperm characteristics and fertility were obtained for motility (r = 0.53; P < 0.01), normal morphology (r = 0.59; P < 0.01) and swollen spermatozoa (r = 0.57; P < 0.01). Analysis of specific sperm swelling patterns showed that those patterns considered to reflect maximal sperm swelling were indicative of high fertility.
本研究使用了来自22头荷斯坦公牛的冷冻精液样本，这些公牛代表了广泛的田间生育水平或返情率（NRR）。精液样本在37摄氏度下解冻至少30秒，然后通过常规精液分析（RSA）和其他精子功能测试进行评估。RSA通过评估精子数量、活力和形态特征来进行。其他精子功能测试通过评估顶体膜完整性、精子穿透宫颈黏液和精子膜功能完整性来进行。在评估精子特征后，将各公牛的生育数据与RSA和功能测试结果进行比较。高生育力和低生育力的公牛在精子数量和渐进性活力方面相似（P>0.05）。RSA和功能测试测量的其他特征在高生育力公牛中显著更高（P<0.05）。各精子特征与公牛生育力之间的相关系数高度显著（P<0.01）。精子特征与生育力之间的最高相关系数出现在活力（r = 0.53；P<0.01）、正常形态（r = 0.59；P<0.01）和肿胀精子（r = 0.57；P<0.01）方面。对特定精子肿胀模式的分析表明，那些被认为反映最大精子肿胀的模式表明生育力高。
