Pescovitz M D, Book B K, Aasted B, Dominguez J, Ezquerra A, Trebichavsky I, Novikov B, Valpotic I, Sver L, Nielsen J, Arn S, Sachs D H, Lunney J K, Boyd P C, Walker J, Lee R, Davis W, Barbosa I R, Zuckermann F, Saalmüller A
Indiana University, Department of Surgery and Microbiology/Immunology, Indianapolis 46202, USA.
Vet Immunol Immunopathol. 1998 Jan 30;60(3-4):251-60. doi: 10.1016/s0165-2427(97)00101-3.
After initial evaluation of the 176 new and 19 control monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop, 57 were assigned to the T-cell/activation marker subgroup. These 57 mAb were further analyzed using flow cytometry on whole blood lymphocytes, splenocytes, Peyer's patch lymphocytes, in vitro cell lines, broncho-alveolar lavage cells, Con A and PHA blasts, fetal cell populations, and by 2-color flow cytometry against mAb to porcine CD2, CD4, and CD8. Finally, the molecular weights of the target antigens were characterized when possible. As a result of these analyses, 23 mAb were distributed into 7 CD clusters. Newly confirmed mAb assignments included: two CD2; one CD4; two CD5; one wCD6; and one wCD25. Three new mAb were found that reacted with wCD8, one of which defined a new epitope, wCD8c. For the first time, mAb against porcine CD3 were identified, including 6 mAb that reacted with three different epitopes. Several new mAb reacted with antigens whose expression varied depending on the activation state of the test cell. These will require further characterization in order to assign a CD number.
在对提交给第二届国际猪CD研讨会的176种新的单克隆抗体（mAb）和19种对照单克隆抗体进行初步评估后，57种被归入T细胞/活化标志物亚组。使用流式细胞术对全血淋巴细胞、脾细胞、派伊尔结淋巴细胞、体外细胞系、支气管肺泡灌洗细胞、刀豆蛋白A和植物血凝素刺激的母细胞、胎儿细胞群体进行进一步分析，并通过双色流式细胞术检测针对猪CD2、CD4和CD8的单克隆抗体。最后，尽可能对靶抗原的分子量进行表征。这些分析的结果是，23种单克隆抗体被归入7个CD簇。新确认的单克隆抗体归属包括：两种CD2；一种CD4；两种CD5；一种wCD6；一种wCD25。发现三种新的与wCD8反应的单克隆抗体，其中一种定义了一个新表位wCD8c。首次鉴定出针对猪CD3的单克隆抗体，包括6种与三种不同表位反应的单克隆抗体。几种新的单克隆抗体与表达取决于测试细胞活化状态的抗原反应。为了确定一个CD编号，这些需要进一步表征。
