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采用有源相位稳定技术的白光干涉全场定量相成像及其在生物样品中的应用。

Full-field quantitative phase imaging by white-light interferometry with active phase stabilization and its application to biological samples.

作者信息

Li Xinhong, Yamauchi Toyohiko, Iwai Hidenao, Yamashita Yutaka, Zhang Haijun, Hiruma Teruo

机构信息

State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, China.

出版信息

Opt Lett. 2006 Jun 15;31(12):1830-2. doi: 10.1364/ol.31.001830.

Abstract

We report a Koehler-illumination-based full-field, actively stabilized, low-coherence phase-shifting interferometer, which is built on a white-light Michelson interferometer. By using a phase-stepping technique we can obtain full-field phase images of the sample. An actively stabilized phase-lock circuit is employed in the system to reduce phase noise. An application to human epithelial cells (HeLa cells) is achieved in our experiment. The advancement of this technique rests in its ability to take images of unstained biological samples quantitatively and on a nanometer scale.

摘要

我们报道了一种基于柯勒照明的全场、主动稳定、低相干相移干涉仪,它基于白光迈克尔逊干涉仪构建。通过使用相移技术,我们可以获得样品的全场相位图像。系统中采用了主动稳定的锁相电路来降低相位噪声。我们的实验实现了该技术在人类上皮细胞(HeLa细胞)上的应用。这项技术的进步在于其能够在纳米尺度上对未染色的生物样品进行定量成像。

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