Choi Youngwoon, Hosseini Poorya, Kang Jeon Woong, Kang Sungsam, Yang Taeseok Daniel, Hyeon Min Gyu, Kim Beop-Min, So Peter T C, Yaqoob Zahid
School of Biomedical Engineering, Korea University, Seoul 02841, South Korea.
Department of Bio-convergence Engineering, Korea University, Seoul 02841, South Korea.
Optica. 2018 Nov;5(11):1468-1473. doi: 10.1364/OPTICA.5.001468. Epub 2018 Nov 15.
Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase microscope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and three-dimensional resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. A reflection phase microscope can potentially elucidate biomechanical mechanisms of pathological and physiological processes.
许多疾病状态都与作为标志物的细胞生物力学变化相关。无标记相显微镜用于量化热驱动的界面波动，从而推断重要的细胞流变学特性。在此，利用光的时空相干性实现了一种具有卓越深度选择性和更高相位灵敏度的高速反射相显微镜。细胞质结构的纳米级运动能够以精细细节和三维分辨率可视化。具体而言，以视频速率观察到了活细胞核膜上发生的自发波动。通过将反射相转换为位移，发现量化核膜波动的灵敏度约为一纳米。反射相显微镜有可能阐明病理和生理过程的生物力学机制。
