Rivero Elena Riet Correa, Nunes Fabio Daumas
Department of Pathology, Health Science Center, Federal University of Santa Catarina.
Braz Oral Res. 2006 Jan-Mar;20(1):21-4. doi: 10.1590/s1806-83242006000100005. Epub 2006 May 22.
Human Papilomaviruses (HPV) are a group of viruses associated with benign and malignant lesions of cutaneous and mucosal epithelia. Some "high risk" HPV types, especially HPV 16 and 18, are strongly correlated with cervical and anogenital cancers and are also related to the genesis of oral squamous cell carcinomas (OSCC). The aim of this work was to investigate the incidence of HPV infection in 40 paraffin-embedded or fresh specimens of OSCC, using PCR amplification of the viral DNA. Literature based primers (GP5+/GP6+) were used in order to amplify HPV DNA from the L1 gene, present in more than 22 types of HPV. A condyloma case with HPV 16 and 18 detected by in situ hybridization was used as a positive control. Amplification of HPV was observed only in the positive control. No squamous cell carcinoma cases showed DNA viral amplification. Absence of HPV DNA amplification by PCR in the analyzed specimens of OSCCs suggests that this virus not always plays a role in the carcinogenesis process. Discrepancy with some studies found in the literature may be related to methodology or population differences.
人乳头瘤病毒（HPV）是一组与皮肤和黏膜上皮的良性及恶性病变相关的病毒。一些“高危”型HPV，尤其是HPV 16和18，与宫颈癌和肛门生殖器癌密切相关，也与口腔鳞状细胞癌（OSCC）的发生有关。本研究的目的是通过病毒DNA的PCR扩增，调查40例OSCC石蜡包埋或新鲜标本中HPV感染的发生率。为了从存在于22种以上HPV类型中的L1基因扩增HPV DNA，使用了基于文献的引物（GP5+/GP6+）。通过原位杂交检测到HPV 16和18的尖锐湿疣病例用作阳性对照。仅在阳性对照中观察到HPV扩增。没有鳞状细胞癌病例显示病毒DNA扩增。在分析的OSCC标本中通过PCR未检测到HPV DNA扩增，这表明该病毒并非总是在致癌过程中起作用。与文献中一些研究结果的差异可能与方法或人群差异有关。
