Marques Marise da Penha Costa, Bussoloti Filho Ivo, Rossi Lia Mara, Andreoli Maria Antonieta, Cruz Natália Oliveira
Faculdade de Ciências Médicas, Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil; Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Faculdade de Ciências Médicas, Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil; Department of Otorhinolaryngology, Irmandade da Santa Casa de Misericórdia de São Paulo, São Paulo, SP, Brazil.
Braz J Otorhinolaryngol. 2015 Nov-Dec;81(6):598-603. doi: 10.1016/j.bjorl.2015.08.007. Epub 2015 Sep 8.
Many epidemiological studies have suggested that human papillomavirus (HPV), especially type 16, is involved in the genesis of squamous cell carcinoma of the oral cavity and oropharynx, especially in young, non-smoking patients; thus, its detection in lesions in this region is important.
To clarify the capacity of the brushing sampling method to detect the presence of HPV in oral or oropharyngeal lesions through polymerase chain reaction (PCR) testing, and to compare the results with those obtained by biopsy.
Prospective study of adult patients with oral or oropharyngeal lesions assessed by PCR, comparing biopsy specimens with samples obtained by the brushing method. The study was approved by the Research Ethics Committee of the institution.
A total of 35 sample pairs were analyzed, but 45.7% of the brushing samples were inadequate (16/35) and, thus, only 19 pairs could be compared. There was agreement of results in 94.7% (18/19) of the pairs, with HPV identified in 16 of them. HPV DNA was detected in 8.6% (3/35) of biopsy and 5.7% (2/35) of brushing samples.
There was no statistically significant difference between the two methods, but the brushing sampling method showed a higher number of inadequate samples, suggesting that it is an unreliable method for surveillance.
许多流行病学研究表明，人乳头瘤病毒（HPV），尤其是16型，与口腔和口咽鳞状细胞癌的发生有关，特别是在年轻的非吸烟患者中；因此，在该区域病变中检测HPV很重要。
通过聚合酶链反应（PCR）检测，阐明刷检采样方法检测口腔或口咽病变中HPV存在的能力，并将结果与活检结果进行比较。
对成年口腔或口咽病变患者进行前瞻性研究，采用PCR评估，将活检标本与刷检方法获得的样本进行比较。该研究得到了机构研究伦理委员会的批准。
共分析了35对样本，但45.7%的刷检样本不合格（16/35），因此只能比较19对。94.7%（18/19）的样本对结果一致，其中16个样本对检测到HPV。活检样本中8.6%（3/35）和刷检样本中5.7%（2/35）检测到HPV DNA。
两种方法之间无统计学显著差异，但刷检采样方法显示不合格样本数量较多，表明它是一种不可靠的监测方法。
