Miwa Norinaga, Kashiwagi Michiko, Kawamori Fumihiko, Masuda Takashi, Sano Yono, Hiroi Midori, Kurashige Hideaki
Shizuoka Institute of Environment and Hygiene, Japan.
Shokuhin Eiseigaku Zasshi. 2006 Apr;47(2):41-5. doi: 10.3358/shokueishi.47.41.
The incidence and levels of Vibrio parahaemolyticus and thermostable direct hemolysin gene (tdh)-positive organisms in retail seafood were determined. The most probable number-polymerase chain reaction (MPN-PCR) method using a PCR procedure targeting the species-specific thermolabile hemolysin gene (tlh) and tdh was used to determine the levels of V. parahaemolyticus and tdh-positive organisms, respectively. In seafood for raw consumption, V. parahaemolyticus was found in four (13.3%) of 30 fish samples, 11 (55.0%) of 20 crustacean samples, and 29 (96.7%) of 30 mollusc samples. Levels of V. parahaemolyticus were below 10(4) MPN/100 g in all fish and crustacean samples tested. However, they were above 10(4) MPN/100 g in 11 (36.7%) of the 30 mollusc samples. In all seafood for raw consumption, the level of tdh-positive organisms was below the limit of detection (< 30 MPN/100 g). In seafood for cooking, V. parahaemolyticus was found in 15 (75.0%) of 20 fish samples, nine (45.0%) of 20 crustacean sample, and 20 (100%) of 20 mollusc samples. Levels of V. parahaemolyticus were above 10(4) MPN/100 g in only three (15.0%) and one (5.0%) of the 20 fish and 20 crustacean samples, respectively. However, they were above 10(4) MPN/100 g in 18 (90.0%) of the 20 mollusc samples. In seven (35.0%) of the 20 mollusc samples, tdh-positive organisms were found and their levels ranged from 3.6x10 to 1.1 x 103 MPN/100 g. From four of seven tdhpositive samples, tdh-positive V. parahaemolyticus was isolated.
测定了零售海鲜中副溶血性弧菌及耐热直接溶血素基因（tdh）阳性菌的发生率和含量水平。采用针对种特异性不耐热溶血素基因（tlh）和tdh的聚合酶链反应（PCR）的最大可能数（MPN-PCR）方法，分别测定副溶血性弧菌和tdh阳性菌的含量水平。在生食海鲜中，30份鱼类样本中有4份（13.3%）、20份甲壳类样本中有11份（55.0%）以及30份软体动物样本中有29份（96.7%）检测出副溶血性弧菌。在所有检测的鱼类和甲壳类样本中，副溶血性弧菌含量低于10⁴MPN/100g。然而，在30份软体动物样本中有11份（36.7%）含量高于10⁴MPN/100g。在所有生食海鲜中，tdh阳性菌含量低于检测限（<30MPN/100g）。在熟食海鲜中，20份鱼类样本中有15份（75.0%）、20份甲壳类样本中有9份（45.0%）以及20份软体动物样本中有20份（100%）检测出副溶血性弧菌。在20份鱼类样本和20份甲壳类样本中，分别仅有3份（15.0%）和1份（5.0%）副溶血性弧菌含量高于10⁴MPN/100g。然而，在20份软体动物样本中有18份（90.0%）含量高于10⁴MPN/100g。在20份软体动物样本中有7份（35.0%）检测出tdh阳性菌，其含量范围为3.6×10至1.1×10³MPN/100g。在7份tdh阳性样本中的4份中，分离出tdh阳性副溶血性弧菌。
