Kyngdon Craig T, Gauci Charles G, Rolfe Rick A, Velásquez Guzmán Jeanette C, Farfán Salazar Marilú J, Verástegui Pimentel Manuela R, Gonzalez Armando E, Garcia Hector H, Gilmanl Robert H, Strugnell Richard A, Lightowlers Marshall W
Veterinary Clinical Centre, The University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia.
J Parasitol. 2006 Apr;92(2):273-81. doi: 10.1645/GE-619R.1.
Taeniid cestodes infect humans and livestock, causing considerable morbidity and mortality, as well as economic loss. Substantial progress has been made toward the production of recombinant vaccines against cysticercosis in livestock animals. Further development of these vaccines would be aided if a reliable in vitro test were available to measure host-protective immune responses in vaccinated animals. Here, we describe in vitro oncosphere-killing assays for the quantification of host-protective serum antibodies against Taenia pisiformis, Taenia ovis, Taenia saginata, and Taenia solium in rabbits, sheep, cattle, and pigs, respectively. Activated oncospheres of T. pisiformis, T. ovis, T. saginata, and T. solium were incubated in vitro in culture medium, test serum, and a source of complement, and oncosphere killing was assessed after 10 days of culture. In vitro oncosphere killing reflected the presence of specific antibody, and the oncosphere-killing assay typically indicated immunity to the homologous parasite that had been determined in vivo. This study describes the first reliable oncosphere-killing assays for T. pisiformis, T. ovis, T. saginata, and T. solium. These assays will be used for further research into the optimization of recombinant vaccines against cysticercosis.
带绦虫纲绦虫感染人类和家畜，会导致相当高的发病率和死亡率，以及经济损失。在生产针对家畜囊尾蚴病的重组疫苗方面已取得了重大进展。如果有可靠的体外试验可用于测量接种疫苗动物的宿主保护性免疫反应，这些疫苗的进一步开发将得到助力。在此，我们分别描述了用于定量兔、绵羊、牛和猪体内针对豆状带绦虫、羊带绦虫、牛带绦虫和猪带绦虫的宿主保护性血清抗体的体外六钩蚴杀伤试验。将豆状带绦虫、羊带绦虫、牛带绦虫和猪带绦虫的活化六钩蚴在含有培养基、试验血清和补体来源的体外环境中培养，培养10天后评估六钩蚴的杀伤情况。体外六钩蚴杀伤反映了特异性抗体的存在，并且六钩蚴杀伤试验通常表明对在体内已确定的同源寄生虫具有免疫力。本研究描述了首例针对豆状带绦虫、羊带绦虫、牛带绦虫和猪带绦虫的可靠六钩蚴杀伤试验。这些试验将用于进一步研究针对囊尾蚴病的重组疫苗的优化。
