Garcia-Allan C, Martínez N, Flisser A, Aluja A, Allan J C, Craig P S
Department of Parasitology, Liverpool School of Tropical Medicine, UK.
J Helminthol. 1996 Dec;70(4):271-80. doi: 10.1017/s0022149x00015558.
A partial immunocharacterization of oncosphere and metacestode antigens of Taenia solium was carried out and compared to antigens from other taeniid species. The results indicated that T. solium metacestode antigen contained epitopes cross reactive with rabbit anti-sera to adult and oncospheral stages of the parasite. Oncospheres, however, consisted largely of stage specific antigens. Western blot analysis indicated that T. solium and T. pisiformis shared several oncospheral antigens; however, this was not the case with T. solium and T. hydatigena. Western blot analysis showed a time-related increase in the number of molecules recognized by antibodies to T. solium oncosphere and metacestode antigens in pigs experimentally infected with T. solium eggs. Oncosphere specific antibodies were detected in pig sera one month after experimental infection whereas antibodies to cystic stage antigens were not present until the 3rd to 5th month post infection. Sera from neurocysticercotic patients as well as naturally infected cysticercotic pigs recognized high molecular weight antigens in the oncospheres.
对猪带绦虫六钩蚴和囊尾蚴抗原进行了部分免疫特性分析，并与其他带科物种的抗原进行了比较。结果表明，猪带绦虫囊尾蚴抗原含有与兔抗该寄生虫成虫和六钩蚴阶段血清发生交叉反应的表位。然而，六钩蚴主要由阶段特异性抗原组成。蛋白质印迹分析表明，猪带绦虫和豆状带绦虫共有几种六钩蚴抗原；然而，猪带绦虫和有钩绦虫的情况并非如此。蛋白质印迹分析显示，在实验感染猪带绦虫卵的猪中，针对猪带绦虫六钩蚴和囊尾蚴抗原的抗体识别的分子数量随时间增加。实验感染后1个月在猪血清中检测到六钩蚴特异性抗体，而直到感染后第3至5个月才出现针对囊尾蚴阶段抗原的抗体。神经囊尾蚴病患者以及自然感染囊尾蚴的猪的血清识别六钩蚴中的高分子量抗原。
