Porcel S, León F, Martín Calderín P, Valero A, Botello A, Alvarez Cuesta E
Department of Allergy, Hospital Ramón y Cajal, Madrid, Spain.
Allergol Immunopathol (Madr). 2001 Sep-Oct;29(5):207-11. doi: 10.1016/s0301-0546(01)79058-1.
although grass pea belongs to the leguminoseae family, allergic reactions to its flour have rarely been described. Clinical and immunological studies were performed to confirm a type I hypersensitivity mechanism in a case of occupational asthma to grass pea flour exposure, used in the industrial processing of parquet.
occupational asthma was diagnosed according to patient history, PEFR monitoring and a specific bronchial challenge test. Skin prick test with an aqueous grass pea flour extract, specific IgE determinations (CAP assay) and IgE immunoblot tests were performed.
skin prick test with the extract showed a positive immediate response, and negative response in controls. Specific IgE to grass pea was positive (9.57 KU/l). Immunoblotting demonstrated the presence of specific serum IgE that recognized 3 proteins in the extract (MW 46, 32 and 28 kDa). PEFR monitoring showed positive results. Bronchial challenge test with the extract elicited an isolated immediate response.
as far as we know this is the first time that IgE mediated occupational asthma caused by grass pea is reported and it is also the first time that its allergens are characterized. Grass pea flour might constitute a relevant occupational allergen in this unreported source of exposure in parquet manufacturers.
尽管草豌豆属于豆科植物，但对其面粉的过敏反应鲜有报道。本研究通过临床和免疫学方法，证实了1例因接触草豌豆粉而导致职业性哮喘的Ⅰ型超敏反应机制，草豌豆粉用于镶木地板的工业加工。
根据患者病史、呼气峰流速（PEFR）监测及特异性支气管激发试验诊断职业性哮喘。采用草豌豆粉水提取物进行皮肤点刺试验、检测特异性IgE（CAP检测法）及IgE免疫印迹试验。
提取物皮肤点刺试验显示即刻阳性反应，而对照为阴性反应。草豌豆特异性IgE呈阳性（9.57 KU/l）。免疫印迹显示血清中存在特异性IgE，可识别提取物中的3种蛋白（分子量分别为46、32和28 kDa）。PEFR监测结果呈阳性。提取物支气管激发试验引发了孤立的即刻反应。
据我们所知，这是首次报道由草豌豆引起的IgE介导的职业性哮喘，也是首次对其过敏原进行鉴定。在镶木地板制造商这种未报道的暴露源中，草豌豆粉可能是一种重要的职业过敏原。
