Rodríguez Alhelí, Solano-Gallego Laia, Ojeda Ana, Quintana Josefina, Riera Cristina, Gállego Montserrat, Portús Montserrat, Alberola Jordi
Departament de Farmacologia, Terapèutica i Toxicologia, Facultat de Veterinària, Universitat Autònoma de Barcelona, Spain.
J Vet Intern Med. 2006 May-Jun;20(3):495-8. doi: 10.1892/0891-6640(2006)20[495:doliii]2.0.co;2.
Concentrations of Leishmania-specific immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA) isotypes were analyzed by enzyme-linked immunosorbent assay (ELISA) in 23 dogs naturally infected with Leishmania infantum before and 1 year after initiating drug therapy. Results showed a high expression and prevalence of Leishmania-specific IgG (176.4 +/- 89 ELISA units [EU]), IgM (105.3 +/- 95.5 EU), and IgA (153.6 +/- 98 EU) in dogs before treatment (median +/- interquartile range EU). One year after treatment was started, dogs were classified as responsive dogs (RDs; n = 13) or unresponsive dogs (UDs; n = 10) based on clinicopathologic findings. Both groups of dogs experienced a statistically significant decrease (P < .05) in Leishmania-specific IgG (RDs = 27%, UDs = 41%), IgM (RDs = 42%, UDs = 29%), and IgA (RDs = 56%, UDs = 46%). Concentrations of specific IgG and IgM were not different at diagnosis or after treatment between the 2 groups. However, the median value for Leishmania-specific IgA 1 year after treatment was significantly lower (P < .05) in RDs (60.8 +/- 67 EU) than in UDs (117 +/- 54 EU). Examination of our data indicates that both the IgA isotype, which is mostly produced by mucosal plasma cells, and the IgM isotype are increased in infected symptomatic dogs, as previously reported for IgG. These 3 isotypes decreased significantly 1 year after initiation of medical treatment.
采用酶联免疫吸附测定（ELISA）法分析了23只自然感染婴儿利什曼原虫的犬在开始药物治疗前及治疗1年后利什曼原虫特异性免疫球蛋白G（IgG）、免疫球蛋白M（IgM）和免疫球蛋白A（IgA）同种型的浓度。结果显示，治疗前犬体内利什曼原虫特异性IgG（176.4±89酶联免疫吸附测定单位[EU]）、IgM（105.3±95.5 EU）和IgA（153.6±98 EU）的表达水平和阳性率较高（中位数±四分位间距EU）。开始治疗1年后，根据临床病理检查结果将犬分为反应性犬（RDs；n = 13）和无反应性犬（UDs；n = 10）。两组犬的利什曼原虫特异性IgG（RDs = 27%，UDs = 41%）、IgM（RDs = 42%，UDs = 29%）和IgA（RDs = 56%，UDs = 46%）均出现统计学显著下降（P < 0.05）。两组在诊断时或治疗后特异性IgG和IgM的浓度无差异。然而，治疗1年后，反应性犬利什曼原虫特异性IgA的中位数（60.8±67 EU）显著低于无反应性犬（117±54 EU）（P < 0.05）。对我们数据的分析表明，如先前关于IgG的报道，主要由黏膜浆细胞产生的IgA同种型和IgM同种型在有症状的感染犬中均升高。开始药物治疗1年后，这3种同种型均显著下降。
