Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan.
Acta Biomater. 2010 Jun;6(6):2140-7. doi: 10.1016/j.actbio.2009.12.023. Epub 2009 Dec 24.
In this study we propose a novel electrospinning fabrication process for the production of a nanofibrous matrix composed of collagen and hyaluronate. This procedure utilized 1,1,1,3,3,3-hexafluoro-2-propanol and formic acid as a mixed solvent. Fluorescence microscopy photographs revealed that the resulting electrospun nanofibers contained both collagen and hyaluronate. The mean diameter of the composite nanofibrous matrix (as observed using scanning electron micrographs) was approximately 200nm; this dimension is similar to that of native fibrous protein within the extracellular matrix. The expression of proteinases (e.g. matrix metalloproteinases, MMPs) and tissue inhibitors of metalloproteinases (TIMPs) have been implicated in epidermal repair during wound healing. Moreover, the characteristics of scarless wounds are known to be related to a decreased ratio of TIMP to MMP expression. In the present study the ratio of expression of TIMP1 to MMP1 was lower in foreskin fibroblast cells that were cultured on a hyaluronate-collagen composite nanofibrous matrix than in those cultured on an exclusively collagen nanofibrous matrix. This indicates that the hyaluronate-collagen composite nanofibrous matrix could potentially be used as a wound dressing for the regeneration of scarless skin.
在这项研究中，我们提出了一种新颖的静电纺丝制造工艺，用于生产由胶原蛋白和透明质酸组成的纳米纤维基质。该方法利用 1,1,1,3,3,3-六氟-2-丙醇和甲酸作为混合溶剂。荧光显微镜照片显示，所得的静电纺纳米纤维含有胶原蛋白和透明质酸。复合纳米纤维基质的平均直径（通过扫描电子显微镜观察）约为 200nm；这个尺寸与细胞外基质中天然纤维蛋白的尺寸相似。蛋白酶（例如基质金属蛋白酶，MMPs）和金属蛋白酶抑制剂（TIMPs）的表达已被认为与伤口愈合过程中的表皮修复有关。此外，无瘢痕伤口的特征已知与 TIMP 与 MMP 表达的比率降低有关。在本研究中，培养在透明质酸-胶原蛋白复合纳米纤维基质上的包皮成纤维细胞中 TIMP1 与 MMP1 的表达比率低于培养在纯胶原蛋白纳米纤维基质上的细胞。这表明透明质酸-胶原蛋白复合纳米纤维基质可潜在地用作无瘢痕皮肤再生的伤口敷料。
