测定T7 DNA的1链5'端的18个核苷酸序列以及r链5'端的15个核苷酸序列。

Nucleic Acids Res. 1975 Jun;2(6):839-52. doi: 10.1093/nar/2.6.839.

The sequences of 18 nucleotides from the 5'-end of the 1-strand and 15 nucleotides from the 5'-end of the r-strand of T7 bacteriophage DNA have been determined to be pT-C-T-C-A-C-A-G-T-G-T-A-C-G-T-C-C-C (1-strand) and pA-G-G-G-A-C-A-C-A-G-C-G-C-T-C (r-strand). The 5'-termini of whole DNA or separated strands were kinased using polynucleotide kinase and (gamma-32-P) rATP. The DNA was partially digested with pancreatic DNase and the fragments were separated by two dimensional electrophoresis and homochromatography. To complete the sequence, snake venom phosphodiesterase digestions of these fragments were carried out. The relationship of these sequences to the proposed cleavage of concatemeric DNA during DNA replication is discussed.

已确定T7噬菌体DNA的1链5'端的18个核苷酸序列和r链5'端的15个核苷酸序列分别为pT-C-T-C-A-C-A-G-T-G-T-A-C-G-T-C-C-C（1链）和pA-G-G-G-A-C-A-C-A-G-C-G-C-T-C（r链）。使用多核苷酸激酶和（γ-32-P）rATP对完整DNA或分离链的5'末端进行激酶处理。用胰脱氧核糖核酸酶对DNA进行部分消化，然后通过二维电泳和同系层析法分离片段。为了完成测序，对这些片段进行了蛇毒磷酸二酯酶消化。讨论了这些序列与DNA复制过程中串联DNA的假定切割之间的关系。