微小RNA的发现方法。

Approaches to microRNA discovery.

作者信息

Berezikov Eugene, Cuppen Edwin, Plasterk Ronald H A

机构信息

Hubrecht Laboratory, Uppsalalaan 8, 3584CT Utrecht, The Netherlands.

出版信息

Nat Genet. 2006 Jun;38 Suppl:S2-7. doi: 10.1038/ng1794.

DOI:10.1038/ng1794

PMID:16736019

Abstract

MicroRNAs (miRNAs) are noncoding RNAs that can regulate gene expression. Several hundred genes encoding miRNAs have been experimentally identified in animals, and many more are predicted by computational methods. How can new miRNAs be discovered and distinguished from other types of small RNA? Here we summarize current methods for identifying and validating miRNAs and discuss criteria used to define an miRNA.

摘要

微小RNA（miRNA）是一类可调控基因表达的非编码RNA。在动物中，通过实验已鉴定出数百个编码miRNA的基因，并且通过计算方法预测出了更多的此类基因。如何发现新的miRNA并将其与其他类型的小RNA区分开来呢？在此，我们总结了目前鉴定和验证miRNA的方法，并讨论了用于定义miRNA的标准。