Department of Pathology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, USA.
The Northport Veteran's Administration Medical Center, Northport, NY, USA.
Methods Mol Biol. 2023;2712:223-232. doi: 10.1007/978-1-0716-3433-2_20.
MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that are involved in a wide range of biological processes, including development, differentiation, and disease. They function by binding to the 3' untranslated region (UTR) of target mRNAs, leading to mRNA degradation or translational repression. miRNAs are involved in the regulation of many cellular processes, including cell proliferation, apoptosis, and metabolism. MiRNAs have been shown to modulate ferroptosis in several ways. Some miRNAs have been shown to promote ferroptosis by increasing the expression of genes involved in lipid peroxidation. Other miRNAs have been shown to inhibit ferroptosis by decreasing the expression of genes involved in iron uptake. The role of miRNAs in ferroptosis is still being studied, but they play a significant role in this cell death pathway. miRNAs may be potential targets for therapeutic intervention in diseases associated with ferroptosis, such as cancer and neurodegenerative diseases. This chapter outlines several methods used to study the connection between miRNAs and ferroptosis through target discovery and validation.
微小 RNA（miRNA）是一类小的非编码 RNA 分子，参与广泛的生物学过程，包括发育、分化和疾病。它们通过与靶 mRNA 的 3'非翻译区（UTR）结合来发挥作用，导致 mRNA 降解或翻译抑制。miRNA 参与调节许多细胞过程，包括细胞增殖、凋亡和代谢。miRNA 已被证明可以通过增加参与脂质过氧化的基因的表达来调节铁死亡。其他 miRNA 已被证明通过降低参与铁摄取的基因的表达来抑制铁死亡。miRNA 在铁死亡中的作用仍在研究中，但它们在这个细胞死亡途径中发挥着重要作用。miRNA 可能是与铁死亡相关疾病（如癌症和神经退行性疾病）治疗干预的潜在靶点。本章概述了通过靶标发现和验证来研究 miRNA 与铁死亡之间联系的几种方法。
