Schütz I, Gus-Mayer S, Schmelzer E
Central Microscopy, Max Planck Institute for Plant Breeding Research, Cologne.
Protoplasma. 2006 May;227(2-4):229-35. doi: 10.1007/s00709-005-0151-1. Epub 2006 May 30.
We have found 5 profilin cDNAs in cultured parsley cells, representing a small gene family of about 5 members in parsley. Specific antibodies were produced using heterologously expressed parsley profilin as antigen. Western blot analysis revealed the occurrence of similar amounts of profilin in roots and green parts of parsley plants. Immunocytochemical staining of parsley cells infected with the oomycetous plant pathogen Phytophthora infestans clearly revealed that profilin accumulates at the site on the plasma membrane subtending the oomycetous appressorium, where the actin cables focus. We also observed the accumulation of Rop GTPases around this site, which might point to a potential function in signaling to the cytoskeleton.
我们在培养的欧芹细胞中发现了5个肌动蛋白结合蛋白的cDNA，这代表了欧芹中一个约有5个成员的小基因家族。以异源表达的欧芹肌动蛋白结合蛋白作为抗原制备了特异性抗体。蛋白质免疫印迹分析显示，欧芹植株的根和绿色部分中肌动蛋白结合蛋白的含量相似。对感染卵菌植物病原体致病疫霉的欧芹细胞进行免疫细胞化学染色，清楚地显示肌动蛋白结合蛋白积聚在卵菌附着胞下方质膜上肌动蛋白丝束集中的部位。我们还观察到Rop鸟苷三磷酸酶在该部位周围的积聚，这可能表明其在向细胞骨架发出信号方面具有潜在功能。
