Ko Jung-Moon, Ju Jongil, Lee Sanghoon, Cha Hyeon-Cheol
Department of Biology, Dankook University, Cheonan, South Korea.
Protoplasma. 2006 May;227(2-4):237-40. doi: 10.1007/s00709-005-0142-2. Epub 2006 May 3.
Several advances have been made in the use of microfluidic devices for insect and mammalian cell cultures, but no reports of their use for plant cell cultures have been published. We, therefore, conducted a plant cell culture in a microfluidic device using polydimethylsiloxane. Nicotiana tabacum protoplasts were cultured in a variously shaped polydimethylsiloxane channel containing Nitsch medium supplemented with 0.5 g of NLN-13 vitamin mixture, 2.0 mg of alpha-naphthaleneacetic acid, and 0.5 mg of 6-benzyladenine per liter and 9% mannitol. Protoplasts in the polydimethylsiloxane channel showed cell division and microcolony formation within 4 weeks. The use of a microfluidic channel is a novel technique in the field of plant cell culture. The results of this study will encourage the utilization of polydimethylsiloxane-based microfluidic devices in plant cell engineering and cell analysis.
在将微流控装置用于昆虫和哺乳动物细胞培养方面已经取得了一些进展，但尚未有关于其用于植物细胞培养的报道。因此，我们使用聚二甲基硅氧烷在微流控装置中进行了植物细胞培养。将烟草原生质体培养在形状各异的聚二甲基硅氧烷通道中，该通道含有每升补充有0.5 g NLN - 13维生素混合物、2.0 mgα-萘乙酸和0.5 mg 6 - 苄基腺嘌呤以及9%甘露醇的Nitsch培养基。聚二甲基硅氧烷通道中的原生质体在4周内显示出细胞分裂和微菌落形成。微流控通道的使用是植物细胞培养领域的一项新技术。本研究结果将促进基于聚二甲基硅氧烷的微流控装置在植物细胞工程和细胞分析中的应用。
