Zhu Lin, Ye Xun, Tang Guangxin, Zhao Nanming, Gong Yandao, Zhao Yuanli, Zhao Jizong, Zhang Xiufang
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China.
J Biomed Mater Res A. 2006 Sep 1;78(3):515-22. doi: 10.1002/jbm.a.30745.
This paper describes efforts to improve implant biocompatibility and durability by applying a hybrid technique using composite oxidation. Pure titanium was used as the substrate material. A porous oxide film as the outer layer was produced by micro-arc oxidation and a dense oxide film as the inner layer was produced by pre-anodic oxidation. In this study, physicochemical characteristics, corrosion test, cell attachment behavior, and in vivo studies were used to compare this gradient layer with untreated titanium. The results revealed that the gradient layer was composed of two layers of oxide films which were made up of rutile and anatase and the surface was porous with calcium and phosphor. The corrosion resistance of the gradient layer was improved remarkably, which was about three times the values for titanium and two times the value for the dense layer. The cell-material interaction study indicated that L929 cells seeded and cultured on the gradient layer appeared to attach well and the rate of proliferation was the greatest. The study in vivo showed that the gradient layer had good biocompatibility. This gradient layer provides a material with high corrosion resistance, bioactivity, and biological properties suitable for tissue engineering applications.
本文描述了通过应用复合氧化的混合技术来提高植入物生物相容性和耐久性的努力。使用纯钛作为基底材料。通过微弧氧化制备作为外层的多孔氧化膜，并通过预阳极氧化制备作为内层的致密氧化膜。在本研究中，利用物理化学特性、腐蚀试验、细胞附着行为和体内研究来将这种梯度层与未处理的钛进行比较。结果表明，梯度层由两层由金红石和锐钛矿组成的氧化膜构成，且表面含有钙和磷，呈多孔状。梯度层的耐腐蚀性显著提高，约为钛的三倍，致密层的两倍。细胞-材料相互作用研究表明，接种并培养在梯度层上的L929细胞似乎附着良好，增殖速率最大。体内研究表明，梯度层具有良好的生物相容性。这种梯度层提供了一种具有高耐腐蚀性、生物活性和适合组织工程应用的生物学特性的材料。
