Niu Li-Li, Liu Xiao-Li, Chen Shuang-Ya, Dong Xiu-Zhu
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Wei Sheng Wu Xue Bao. 2006 Apr;46(2):280-4.
An anaerobic, thermophilic, hydrogen-producing strain T42 was obtained from a hot spring of South Mountain District, Tibet. Cells are Gram-positive, mobile rod-shaped. Spores were not observed. Temperature range for growth is 32 degrees C to 69 degrees C (optimum temperature, 60 degrees C - 62 degrees C), and pH range for growth is 5.0 to 8.8 (optimum pH, 7.0 - 7.5). The generation time is around 30 min. Organic nitrogen sourc is required for growth. Strain T42 utilizes a wide range of carbohydrates, including starch, dextrin, sucrose, cellobiose, fructose, maltose, ribose, glycogen and galactose. Acetate, ethanol, H2 and CO2 are the end products of glucose fermentation. The (G + C) content of strain T42 is 31.2 mol%. Phylogenetic analysis based on the 16S rDNA sequence similarity indicates that strain T42 is the closest relative to Thermobrachium celere and Caloramator indicus. Biological characteristics and phylogenetic analysis of the 16S rDNA gene indicate the new strain belongs to the genus Thermobrachium. Strain T42 produces H2 from glucose at maximal level when growing at 62 degrees C and initial pH 7.2, the hydrogen yields and maximal hydrogen production rate are 1.06 mol H2/mol glucose and 24.0 mmol H2/gDW/h, respectively. Strain T42 also produced H2 by fermentating from a variety of carbohydrates. 20 mmol/L Magnesium and 2 mmol/L iron increase the hydrogen production content by 20% and 23.3%, respectively, but nickel has no effect on the hydrogen production. In the co-culture of strain T42 and methane-producing strain M. thermautotrophicus Z245, hydrogen pressure is dramatically decreased, meanwhile deduced H2 production and the consumption of glucose are increased markedly by 2.8 fold and 1 fold, and the ratio of acetate/ethanol is enhanced froml to 1.7.
从西藏南山地区的一处温泉中获得了一株厌氧、嗜热、产氢菌株T42。细胞为革兰氏阳性、可运动的杆状。未观察到孢子。生长温度范围为32℃至69℃（最适温度60℃ - 62℃），生长pH范围为5.0至8.8（最适pH 7.0 - 7.5）。代时约为30分钟。生长需要有机氮源。菌株T42利用多种碳水化合物，包括淀粉、糊精、蔗糖、纤维二糖、果糖、麦芽糖、核糖、糖原和半乳糖。乙酸盐、乙醇、H2和CO2是葡萄糖发酵的终产物。菌株T42的（G + C）含量为31.2 mol%。基于16S rDNA序列相似性的系统发育分析表明，菌株T42与嗜热短臂菌（Thermobrachium celere）和印度热聚菌（Caloramator indicus）亲缘关系最近。16S rDNA基因的生物学特性和系统发育分析表明该新菌株属于嗜热短臂菌属。菌株T42在62℃和初始pH 7.2条件下生长时，从葡萄糖产生H2的水平最高，氢气产率和最大产氢速率分别为1.06 mol H2/mol葡萄糖和24.0 mmol H2/gDW/h。菌株T42也通过发酵多种碳水化合物产生H2。20 mmol/L的镁和2 mmol/L的铁分别使产氢量增加20%和23.3%，但镍对产氢没有影响。在菌株T42与产甲烷菌株嗜热自养甲烷杆菌（M. thermautotrophicus）Z245的共培养中，氢气压力显著降低，同时推测的H2产量和葡萄糖消耗量分别显著增加2.8倍和1倍，乙酸盐/乙醇的比例从1提高到1.7。
