Song Lei, Dong Xiuzhu
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2132-5. doi: 10.1099/ijs.0.65635-0.
A Gram-stain-positive, strictly anaerobic, mesophilic, amylolytic, rod-shaped bacterium, designated strain SW408(T), was isolated from a laboratory-scale H(2)-producing upflow anaerobic sludge blanket reactor. The strain grew at 24-45 degrees C (no growth at or below 22 degrees C or at or above 47 degrees C), with optimum growth at 37 degrees C. The pH range for growth was 4.0-9.0 (no growth at or below pH 3.6 or at or above pH 9.3), with optimum growth at pH 7.0. Starch, cellobiose, glucose, fructose, galactose, lactose, maltose, mannose, ribose and sucrose supported growth. The major end products from glucose fermentation were ethanol, acetate, hydrogen and carbon dioxide. Abundant H(2) was produced from starch fermentation. The DNA G+C content was 33.1 mol% (T(m) method). Phylogenetic analysis based on 16S rRNA gene sequence analysis showed that the bacterium represents a previously unrecognized species within Clostridium rRNA cluster I and is most closely related to the type strain of Clostridium frigidicarnis (94.9% similarity). On the basis of phenotypic, genotypic and phylogenetic characteristics, strain SW408(T) was identified as a representative of a novel species of the genus Clostridium, for which the name Clostridium amylolyticum sp. nov. is proposed. The type strain is SW408(T) (=JCM 14823(T)=AS 1.5069(T)=CGMCC 1.5069(T)).
从实验室规模的产氢上流式厌氧污泥床反应器中分离出一株革兰氏阳性、严格厌氧、嗜温、解淀粉、杆状细菌，命名为SW408(T)菌株。该菌株在24 - 45℃生长（22℃及以下或47℃及以上不生长），最适生长温度为37℃。生长的pH范围为4.0 - 9.0（pH 3.6及以下或pH 9.3及以上不生长），最适生长pH为7.0。淀粉、纤维二糖、葡萄糖、果糖、半乳糖、乳糖、麦芽糖、甘露糖、核糖和蔗糖支持生长。葡萄糖发酵的主要终产物是乙醇、乙酸、氢气和二氧化碳。淀粉发酵产生大量氢气。DNA G + C含量为33.1 mol%（Tm法）。基于16S rRNA基因序列分析的系统发育分析表明，该细菌代表梭菌属rRNA簇I内一个以前未被认识的物种，与冷肉梭菌模式菌株关系最密切（相似性为94.9%）。根据表型、基因型和系统发育特征，SW408(T)菌株被鉴定为梭菌属一个新物种的代表，为此提出新种名为解淀粉梭菌。模式菌株为SW408(T)（=JCM 14823(T)=AS 1.5069(T)=CGMCC 1.5069(T)）。
