Yuan Zhi-Hui, Lan Xi-Qian, Yang Ting, Xiao Jie, Zhou Ze-Yang
Sericultural Key Laboratory of Agricultural Ministry, Southwest University, Chongqing 400716, China.
Wei Sheng Wu Xue Bao. 2006 Apr;46(2):285-91.
Majority of the environmental microorganisms are still unculturable. To shed a light on the bacteria community in the silkworm intestine more completely, culture-dependent and culture-independent methods were used to perform the investigation. As the latter, 16s rRNA gene was amplified and a library was constructed by using the meta-genomic DNA extracted from the bacteria in the silkworm intestine as template. Restriction fragment length polymorphism (RFLP) method was used to screen recombinants originated from different bacteria, and the nucleotide sequences were determined. A BLAST searching in the GenBank and an analysis on phylogenesis were performed. The taxonomy of these bacteria and their physiological function to silkworm were speculated. Results showed that the bacteria in silkworm intestine are mainly belong to the genera Arthrobacter, Lactobacillus, Pseudomonas, Escherichia, Micrococcus, Bacillus, and Staphylococcus. They may play important roles to the host silkworm in the utilization of their food mulberry and disease prevention. Both the culture-dependent and culture-independent methods have their advantages and disadvantages, and they are complemented with each other.
大多数环境微生物仍无法培养。为了更全面地了解家蚕肠道中的细菌群落，采用了依赖培养和不依赖培养的方法进行研究。作为后者，以从家蚕肠道细菌中提取的宏基因组DNA为模板，扩增16s rRNA基因并构建文库。采用限制性片段长度多态性（RFLP）方法筛选来自不同细菌的重组体，并测定核苷酸序列。在GenBank中进行BLAST搜索并进行系统发育分析。推测了这些细菌的分类及其对家蚕的生理功能。结果表明，家蚕肠道中的细菌主要属于节杆菌属、乳杆菌属、假单胞菌属、大肠杆菌属、微球菌属、芽孢杆菌属和葡萄球菌属。它们可能在宿主家蚕利用其食物桑叶和预防疾病方面发挥重要作用。依赖培养和不依赖培养的方法都有其优缺点，且相互补充。
