Li Ke, Zheng Tian-ling, Tian Yun, Yuan Jian-jun
Institute of Applied and Environmental Microbiology, School of Life Sciences, Xiamen University, Xiamen 361005, China.
Wei Sheng Wu Xue Bao. 2007 Aug;47(4):649-53.
The composition of bacterial community in the intestine of the white shrimp, Litopenaeus vannamei under laboratory culture condition was determined using the 16S rDNA clone library. 16s rRNA gene was amplified and a library was constructed by using the genomic DNA extracted from the bacteria in the shrimp intestine as template. 12 different RFLP patterns of the clones were obtained by restriction fragment length polymorphism analysis using Afa I and Msp I. Compared with the published sequences in GenBank database, sequencing results of cloned 16S rDNA amplicons revealed a diverse community including gamma-proteobacteria and Firmicutes in the intestine of artificial diet-fed shrimp. Results showed that the Firmicutes group can be a dominant component (75.4%) in the shrimp intestinal microflora and other clones belong to gamma-proteobacteria (24.6%) which were identified as Shewanella sp., Pantoea sp., Aranicola sp., Pseudomonas sp. and Vibrio sp., respectively. These results provide the first comprehensive description of microbial diversity of the white shrimp intestine and suggest that most of the bacteria associated with shrimp intestine are uncultured and novel species.
采用16S rDNA克隆文库法测定了实验室养殖条件下南美白对虾肠道细菌群落的组成。以对虾肠道细菌提取的基因组DNA为模板，扩增16s rRNA基因并构建文库。利用Afa I和Msp I进行限制性片段长度多态性分析，获得了12种不同的克隆RFLP图谱。通过与GenBank数据库中已发表序列的比对，克隆的16S rDNA扩增子测序结果显示，人工饲料喂养的对虾肠道内存在一个包括γ-变形菌门和厚壁菌门的多样化群落。结果表明，厚壁菌门在对虾肠道微生物区系中可能是优势菌群（75.4%），其他克隆分别属于γ-变形菌门（24.6%），鉴定为希瓦氏菌属、泛菌属、阿拉尼科拉菌属、假单胞菌属和弧菌属。这些结果首次全面描述了南美白对虾肠道微生物的多样性，并表明与对虾肠道相关的大多数细菌是未培养的新物种。
