Kay P H, Jacobsen P F, Zheng M H, Papadimitriou J M
Department of Pathology, University of Western Australia, Nedlands.
Gene. 1991 Mar 15;99(2):273-7. doi: 10.1016/0378-1119(91)90138-2.
Polymorphism of the myogenic gene, Myo-D1, has been sought to examine genetic mechanisms which control skeletal muscle development. By Southern analysis, three restriction-fragment length polymorphisms (RFLPs) have been found in various mouse strains using the TaqI, SacI and BglII restriction endonucleases and a full-length cDNA Myo-D1 probe. Reference to the distribution of RFLPs in different mouse strains derived from Mus mus (M.m.) domesticus and M.m. musculus subspecies suggests that Myo-D1 rearrangements are subject to nonrandom association. The biological significance of RFLP of the Myo-D1 gene is yet to be determined.
人们一直在寻找生肌基因Myo-D1的多态性，以研究控制骨骼肌发育的遗传机制。通过Southern分析，使用TaqI、SacI和BglII限制性内切酶以及全长cDNA Myo-D1探针，在各种小鼠品系中发现了三种限制性片段长度多态性（RFLP）。参考源自小家鼠（M.m.）和小鼠（M.m.）亚种的不同小鼠品系中RFLP的分布表明，Myo-D1重排存在非随机关联。Myo-D1基因RFLP的生物学意义尚待确定。
