Watanabe T, Miyashita N, Nishimura M, Saitou N, Hayashi Y, Moriwaki K
Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, Japan.
Biochem Genet. 1989 Feb;27(1-2):119-30. doi: 10.1007/BF00563022.
Restriction endonuclease fragment length variants in mice were compared by Southern blot analysis using the cDNA probe pcXP33 for the chymotrypsin gene. The variants were detected in the restriction patterns generated by fragments from digestions with BglII, EcoRI, HindIII, Pstl, SacI, and XbaI. The set of protein phenotypes and the restriction patterns of chymotrypsin gene were examined in many laboratory strains and wild subspecies. Most laboratory strains (26 strains) are grouped into a set defined as Set 1, but only a few laboratory strains (AU/SsJ and five BALB/c sublines) are classified as belonging to Set 2. Of wild subspecies, only BRV-MPL (M. brevirostris) can be placed in Set 1, while DOM-BLG and SK/Cam (M. domesticus) belong in Set 2. The assignment of an appropriate set defined by the characteristics of the chymotrypsin gene has also been investigated in M. musculus, two Chinese subspecies, M. yamashinai, M. molossinus, and M. castaneus, and the evolutionary relationship between laboratory mice and various subspecies of Mus has been examined.
使用胰凝乳蛋白酶基因的cDNA探针pcXP33，通过Southern印迹分析比较了小鼠中的限制性内切酶片段长度变体。在用BglII、EcoRI、HindIII、Pstl、SacI和XbaI消化产生的片段所形成的限制性图谱中检测到了这些变体。在许多实验室品系和野生亚种中检查了胰凝乳蛋白酶基因的蛋白质表型集和限制性图谱。大多数实验室品系（26个品系）被归为定义为集合1的一组，但只有少数实验室品系（AU/SsJ和五个BALB/c亚系）被归类为属于集合2。在野生亚种中，只有BRV-MPL（短尾小鼠）可归入集合1，而DOM-BLG和SK/Cam（小家鼠）属于集合2。还在小家鼠、两个中国亚种、山原小鼠、日本小家鼠和栗色小鼠中研究了根据胰凝乳蛋白酶基因特征定义的合适集合的归属，并研究了实验室小鼠与小家鼠各种亚种之间的进化关系。
