Mattar Rosiane, de Souza Eduardo, Daher Silvia
Department of Obstetrics, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
J Reprod Med. 2006 Apr;51(4):317-20.
To investigate the association of preterm delivery with polymorphisms of IL-6, IL-10, IFN-gamma, TGFbeta1 and TNF-alpha genes.
The study group consisted of 45 Caucasian, 81 mixed race and 13 black women with a history of preterm labor, consecutively referred. All of them had delivered before 37 weeks' gestation. The control group was composed of 56 Caucasian, 48 mixed race and 15 black women with successful pregnancy. DNA was extracted from whole blood, and cytokine genotyping was performed using the Cytokine Genotyping Tray (One-Lambda, Canoba Park, California). The polymorphisms analyzed were: TNF-alpha (-08 G --> A), IL-10 (-1082 G --> A), IL-6 (-174 G --> C), TGFbeta1 (+10 T --> C e 25 C --> G) and IFN-gamma (+874 A --> T).
There were no differences in genotype frequencies of IL-10, TGF-beta, TNF-alpha or IL-6 polymorphisms between the groups. In the Caucasian group there was a trend toward increased frequencies of the TT genotype of IFN-gamma in controls.
Preterm delivery is not associated with TNF-alpha (-308), IL-10 (-1082), IL-6 (-174), TGFbeta1 (+10 e 25) or IFN-gamma (+874) polymorphisms.
研究早产与白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、γ干扰素（IFN-γ）、转化生长因子β1（TGFβ1）和肿瘤坏死因子-α（TNF-α）基因多态性之间的关联。
研究组由45名白种人、81名混血人种和13名有早产史的黑人女性组成，她们是连续转诊而来的。所有这些女性均在妊娠37周前分娩。对照组由56名白种人、48名混血人种和15名成功妊娠的黑人女性组成。从全血中提取DNA，并使用细胞因子基因分型试剂盒（One-Lambda公司，加利福尼亚州卡诺巴帕克）进行细胞因子基因分型。分析的多态性包括：TNF-α（-308 G→A）、IL-10（-1082 G→A）、IL-6（-174 G→C）、TGFβ1（+10 T→C和25 C→G）以及IFN-γ（+874 A→T）。
两组之间IL-10、转化生长因子-β（TGF-β）、TNF-α或IL-6多态性的基因型频率没有差异。在白种人群体中，对照组中IFN-γ的TT基因型频率有增加的趋势。
早产与TNF-α（-308）、IL-10（-1082）、IL-6（-174）、TGFβ1（+10和25）或IFN-γ（+874）多态性无关。
