白细胞介素 10 基因多态性（-819C>T、-592C>A 和-1082G>A）与早产的相关性研究。
Association of IL-10 Gene Polymorphism (-819C > T, -592C > A and -1082G > A) with Preterm Birth.
机构信息
Department of Pediatrics, King George's Medical University, Lucknow, Uttar Pradesh, 226003, India.
Department of Obstetrics and Gynecology, King George's Medical University, Lucknow, Uttar Pradesh, India.
出版信息
Indian J Pediatr. 2018 Feb;85(2):93-101. doi: 10.1007/s12098-017-2496-9. Epub 2017 Nov 17.
OBJECTIVE
To analyze the association of IL-10 gene and its polymorphisms with preterm birth (PTB).
METHODS
Five hundred and fifty nine women with term birth and 559 with preterm birth were recruited from Lucknow, India. Genetic association analysis was conducted between cases and controls. Subjects recruited as cases were women (aged between 18-40 y) with singleton delivery before 37 wk of gestation and controls were with delivery after or on 37 wk. The genotyping was performed for rs1800871, rs1800872 and rs1800896 for assessing the allelic distribution, haplotypic association and linkage disequilibrium analysis. IL-10mRNA levels were evaluated by real time quantitative polymerase chain reaction (PCR) method.
RESULTS
The risk of PTB was found significant in women carrying IL-10 (-1082) GA genotype [OR=1.72(1.7-2.5), p=0.006]. The haplotypic analysis of studied polymorphisms for rs1800871, rs1800872 and rs1800896 depicted the association of ATA (p=0.02) and ATC (p=0.01) haplotypes with PTB. The IL-10 mRNA levels were significantly lower in cases (p=0.05).
CONCLUSIONS
IL-10 marks a protective impact in the inflammatory pathway of PTB.
目的
分析白细胞介素 10（IL-10）基因及其多态性与早产（PTB）的关系。
方法
在印度勒克瑙招募了 559 名足月分娩的妇女和 559 名早产的妇女。对病例和对照组进行了基因关联分析。病例组招募的受试者为年龄在 18-40 岁之间的单胎分娩且妊娠 37 周前分娩的女性，对照组为 37 周后或 37 周时分娩的女性。对 rs1800871、rs1800872 和 rs1800896 进行基因分型，以评估等位基因分布、单倍型关联和连锁不平衡分析。采用实时定量聚合酶链反应（PCR）方法评估 IL-10mRNA 水平。
结果
携带 IL-10（-1082）GA 基因型的妇女发生 PTB 的风险显著增加[比值比（OR）=1.72（1.7-2.5），p=0.006]。对 rs1800871、rs1800872 和 rs1800896 研究多态性的单倍型分析显示，ATA（p=0.02）和 ATC（p=0.01）单倍型与 PTB 相关。病例组的 IL-10mRNA 水平明显降低（p=0.05）。
结论
IL-10 对 PTB 的炎症途径具有保护作用。
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