Veronesi M C, Carluccio A, Kindahl H, Faustini M, Battocchio M, Cairoli F
Dipartimento di Scienze Cliniche Veterinarie - Sez. Clinica Ostetrica e Ginecologica Veterinaria, Milan, Italy.
J Vet Med A Physiol Pathol Clin Med. 2006 Jun;53(5):259-62. doi: 10.1111/j.1439-0442.2006.00820.x.
The aim of the present study was to evaluate within 24 h post-ovulation oxytocin-induced PGF(2alpha) release in mares with and without post-breeding delayed uterine clearance (DUC). Twenty-one of 34 mares with a variable amount of intrauterine fluids accumulation were considered to be affected by delayed uterine clearance (DUC group), while the other 13 mares did not show any uterine fluid accumulation, and were considered as controls (WDUC group). Both DUC and WDUC mares were administered with 20 IU oxytocin i.m. 90 min after the ultrasound examination performed 24 h after breeding. Immediately before, 5 and 10 min after oxytocin administration, blood samples were collected for 15-ketodihydro-PGF(2alpha) (PG-metabolite), 17beta-estradiol, and progesterone analysis. Ultrasonography performed 24 h after oxytocin treatment showed a complete uterine clearance in all DUC mares. The oxytocin-induced PG-metabolite increase was detected in 71.4% DUC mares compared with 38.5% in WDUC group, with a positive trend of release, as evidenced from 5 min after oxytocin administration. In WDUC mares, no significant differences in oxytocin-induced PG-metabolite trend of release were observed. In conclusion, the results of the present study showed the importance of PGF(2alpha) involvement in the pathogenesis of post-breeding DUC in the mare.
本研究的目的是评估排卵后24小时内，催产素诱导的前列腺素F2α（PGF(2α)）在有或无配种后子宫清除延迟（DUC）的母马中的释放情况。34匹子宫内有不同程度积液的母马中，21匹被认为患有子宫清除延迟（DUC组），而另外13匹母马未出现任何子宫积液，被视为对照组（WDUC组）。在配种后24小时进行超声检查90分钟后，DUC组和WDUC组的母马均肌肉注射20 IU催产素。在催产素给药前、给药后5分钟和10分钟，采集血样用于分析15-酮二氢-PGF(2α)（PG代谢物）、17β-雌二醇和孕酮。催产素治疗24小时后进行的超声检查显示，所有DUC组母马的子宫均已完全清除。DUC组71.4%的母马检测到催产素诱导的PG代谢物增加，而WDUC组为38.5%，从催产素给药后5分钟开始呈现出阳性释放趋势。在WDUC组母马中，未观察到催产素诱导的PG代谢物释放趋势有显著差异。总之，本研究结果表明PGF(2α)参与母马配种后DUC发病机制的重要性。
