Moelling Karin, Abels Susanne, Jendis Joerg, Matskevich Alexey, Heinrich Jochen
Institute of Medical Virology, University of Zurich, Switzerland.
FEBS Lett. 2006 Jun 12;580(14):3545-50. doi: 10.1016/j.febslet.2006.05.033. Epub 2006 May 22.
We describe inhibition of HIV replication by a partially double-stranded 54mer oligodeoxynucleotide, ODN, which consists of an antisense strand targeting the highly conserved polypurine tract, PPT, of HIV, and a second strand, compatible with triple-helix formation. Upon treatment of HIV-infected cells with ODN early after infection no viral nucleic acids, syncytia or p24 viral antigen expression was observed. The ODN-mediated effect was highly sequence-specific. The ODN against HIV-IIIB was effective preferentially against its homologous PPT and less against the PPT of HIV-BaL differing in two of 24 nucleotides and vice versa. It may be interesting mechanistically as an antiviral drug.
我们描述了一种部分双链的54聚体寡脱氧核苷酸（ODN）对HIV复制的抑制作用，该ODN由一条靶向HIV高度保守的多聚嘌呤序列（PPT）的反义链和第二条与三链体形成相容的链组成。在感染后早期用ODN处理HIV感染的细胞时，未观察到病毒核酸、多核体或p24病毒抗原表达。ODN介导的作用具有高度序列特异性。针对HIV-IIIB的ODN优先对其同源PPT有效，而对24个核苷酸中有两个不同的HIV-BaL的PPT效果较差，反之亦然。作为一种抗病毒药物，其作用机制可能很有趣。
