Wright André-Denis Girard
CSIRO Livestock Industries, Centre for Environment and Life Sciences, Private Bag 5, Wembley, Western Australia 6913, Australia.
Int J Syst Evol Microbiol. 2006 Jun;56(Pt 6):1223-1227. doi: 10.1099/ijs.0.63776-0.
Phylogenetic relationships within the halophilic archaea were inferred from comparisons of the 16S rRNA gene sequences from 61 strains, representing 18 genera with validly published names within the order Halobacteriales. Trees produced using distance-matrix (least-squares and neighbour-joining) methods affirm with strong bootstrap support that the members of the order Halobacteriales are a monophyletic group. Ten genera were supported as monophyletic groups [Haloarcula, Halobiforma, Halococcus, Haloferax, Halorubrum, Halosimplex (multiple sequences from a single strain), Natrialba, Natrinema, Natronococcus and Natronorubrum] and eight genera were represented by a single strain (Halobacterium, Halobaculum, Halogeometricum, Halomicrobium, Halorhabdus, Halosimplex, Natronobacterium and Natronomonas). The genus Haloterrigena was always paraphyletic, and the phylogenetic placement of and sister groups to Halobacterium and Natronomonas could not be resolved. Both treeing methods failed to resolve the deep branching patterns within the order Halobacteriales and the relationships between the major clades. Additional representation from the currently monospecific genera and/or the use of other macromolecules may be required to resolve the relationships within the order Halobacteriales.
通过比较61个菌株的16S rRNA基因序列推断嗜盐古菌内部的系统发育关系，这些菌株代表了嗜盐杆菌目内18个有效发表名称的属。使用距离矩阵（最小二乘法和邻接法）方法构建的树在强大的自展支持下证实，嗜盐杆菌目成员是一个单系群。10个属被支持为单系群[嗜盐碱杆菌属、盐形菌属、盐球菌属、嗜盐富球菌属、嗜盐红菌属、盐单胞菌属（来自单个菌株的多个序列）、嗜盐碱球菌属、嗜盐碱红菌属、嗜盐碱芽孢杆菌属和嗜盐碱红球菌属]，8个属由单个菌株代表（嗜盐杆菌属、盐杆菌属、盐几何菌属、盐微菌属、盐栖芽孢杆菌属、盐单胞菌属、嗜盐碱杆菌属和嗜盐碱单胞菌属）。嗜盐土芽孢杆菌属总是并系的，嗜盐杆菌属和嗜盐碱单胞菌属的系统发育位置及其姐妹群无法确定。两种建树方法都未能解决嗜盐杆菌目内的深层分支模式以及主要进化枝之间的关系。可能需要目前单种属的更多代表性菌株和/或使用其他大分子来解决嗜盐杆菌目内的关系。
