从中部安纳托利亚（土耳其）盐矿中分离耐盐古菌的特性、鉴定和系统发育分析。

Isolation, characterization and phylogenetic analysis of halophilic archaea from a salt mine in central Anatolia (Turkey).

机构信息

Mustafa Kemal University, Biology Department, 31040 Hatay, Turkey.

出版信息

Pol J Microbiol. 2012;61(2):111-7.

Abstract

The haloarchaeal diversity of a salt mine, a natural cave in central Anatolia, was investigated using convential microbiological and molecular biology methods. Eight halophilic archaeal isolates selected based on their colony morphology and whole cell protein profiles were taxonomically classified on the basis of their morphological, physiological, biochemical properties, polar lipid and protein profiles and 16S rDNA sequences. From the 16S rDNA sequences comparisons it was established that the isolates CH2, CH3 and CHC resembled Halorubrum saccharovorum by 98.8%, 98.9% and 99.5%, respectively. There was a 99.7% similarity between the isolate CH11 and Halobacterium noricense and 99.2% between the isolate CHA1 and Haloarcula argentinensis. The isolate CH8K and CH8B revealed a similarity rate of 99.8% and 99.3% to Halococcus dombrowskii, respectively. It was concluded that the isolates named CH2, CH3 and CHC were clustered in the genus Halorubrum and that CHA1 and CH7 in the genus Haloarcula, CH8K and CH8B in the genus Halococcus and CH11 in the genus Halobacterium.

摘要

采用常规微生物学和分子生物学方法研究了安纳托利亚中部一个天然洞穴盐矿中的盐古菌多样性。根据菌落形态和全细胞蛋白图谱，选择了 8 个嗜盐古菌分离株，根据形态学、生理学、生物化学特性、极性脂质和蛋白质图谱以及 16S rDNA 序列对其进行了分类。从 16S rDNA 序列比较来看，分离株 CH2、CH3 和 CHC 分别与 Halorubrum saccharovorum 的相似度为 98.8%、98.9%和 99.5%。分离株 CH11 与 Halobacterium noricense 的相似度为 99.7%，CHA1 与 Haloarcula argentinensis 的相似度为 99.2%。分离株 CH8K 和 CH8B 与 Halococcus dombrowskii 的相似度分别为 99.8%和 99.3%。研究结果表明，命名为 CH2、CH3 和 CHC 的分离株聚类在 Halorubrum 属中，而 CHA1 和 CH7 聚类在 Haloarcula 属，CH8K 和 CH8B 聚类在 Halococcus 属，CH11 聚类在 Halobacterium 属。