Di Gregorio P, Rando A, Pieragostini E, Masina P
Istituto di Produzione Animale, Facoltà di Agraria, Università della Basilicata, Italy.
Anim Genet. 1991;22(1):21-30. doi: 10.1111/j.1365-2052.1991.tb00643.x.
By using seven endonucleases and four bovine cDNA probes specific for alpha S1-, alpha S2-, beta-, and kappa-casein genes, nine restriction fragment length polymorphisms (RFLPs) have been found in the sheep orthologous DNA regions. In contrast to the low level of variation observed at the protein level, these DNA polymorphisms determine a high level of heterozygosity and, therefore, represent useful tools for genetic analyses since they can also be obtained without the need for gene expression. In fact, informative matings suggest that in sheep, as in cattle, the four loci are linked.
通过使用七种核酸内切酶和四种针对αS1-、αS2-、β-和κ-酪蛋白基因的牛cDNA探针，在绵羊的直系同源DNA区域发现了九种限制性片段长度多态性（RFLP）。与在蛋白质水平观察到的低变异水平相反，这些DNA多态性决定了高杂合度，因此是遗传分析的有用工具，因为无需基因表达即可获得这些多态性。实际上，信息交配表明，与牛一样，绵羊中的四个基因座是连锁的。
