Montgomery G W, Sise J A, Penty J M, Tou H M, Hill D F
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Anim Genet. 1992;23(5):411-6. doi: 10.1111/j.1365-2052.1992.tb02159.x.
A selection of cattle, human and sheep cDNA probes were screened against sheep genomic DNA, cut with 10 different restriction enzymes, to assess the usefulness of these probes for restriction fragment length polymorphism (RFLP) linkage studies in sheep. Two-thirds of the cattle cDNA probes showed moderate to strong homology with sheep DNA samples, compared with less than half of the human cDNA probes at the final washing stringency chosen for the experiments. The set of probes tested detected a useful frequency of RFLPs. Fifty-seven per cent of probes showing moderate to strong homology identified RFLPs with one or more restriction enzymes. Restriction enzymes that detected RFLPs most frequently in sheep were TaqI and MspI. The results show that sheep and cattle cDNA probes, including candidate genes for production traits, identified a high frequency of RFLPs suitable for genetic mapping in sheep.
选用牛、人及绵羊的cDNA探针，与用10种不同限制性内切酶切割的绵羊基因组DNA进行杂交，以评估这些探针在绵羊限制性片段长度多态性（RFLP）连锁研究中的实用性。在实验选择的最终洗涤严谨度下，三分之二的牛cDNA探针与绵羊DNA样本表现出中度至高度同源性，相比之下，人cDNA探针只有不到一半表现出这样的同源性。所测试的探针组检测到了有用频率的RFLP。表现出中度至高度同源性的探针中，57%与一种或多种限制性内切酶鉴定出RFLP。在绵羊中最常检测到RFLP的限制性内切酶是TaqI和MspI。结果表明，绵羊和牛的cDNA探针，包括生产性状的候选基因，鉴定出了适合绵羊遗传图谱构建的高频率RFLP。
