绵羊连锁图谱：用于比较图谱研究的RFLP标记

Sheep linkage mapping: RFLP markers for comparative mapping studies.

作者信息

Montgomery G W, Penty J M, Henry H M, Sise J A, Lord E A, Dodds K G, Hill D F

机构信息

Department of Biochemistry, University of Otago, Dunedin, New Zealand.

出版信息

Anim Genet. 1995 Aug;26(4):249-59. doi: 10.1111/j.1365-2052.1995.tb03252.x.

DOI:10.1111/j.1365-2052.1995.tb03252.x

PMID:7661397

Abstract

Restriction fragment length polymorphisms (RFLPs) detected using cDNA probes for conserved genes provide an important set of markers that anchor or link syntenic groups in a range of divergent mammalian species. DNA probes from sheep, cattle, pig, human and mouse were screened against sheep DNA samples and 24 new RFLP markers for sheep were identified. Among the loci tested, 22 had a homologue that has been mapped in humans. An RFLP for fibronectin (FN1) was linked to alpha-inhibin (INHA) at a distance of 5cM. The FN1 locus has been assigned to sheep chromosome 2q41-q44 and linkage between FN1 and INHA assigns INHA to the same chromosome in sheep. In addition to the new loci reported here, 28 RFLPs have been published previously by this group and these are collated together with RFLPs published from other laboratories. RFLPs have been reported for 86 loci in sheep. Fifty-four loci have been mapped to 16 different chromosomes.

摘要

使用保守基因的cDNA探针检测到的限制性片段长度多态性（RFLP）提供了一组重要的标记，这些标记可在一系列不同的哺乳动物物种中锚定或连接同线群。用绵羊、牛、猪、人类和小鼠的DNA探针筛选绵羊DNA样本，鉴定出24个新的绵羊RFLP标记。在所测试的基因座中，有22个具有已在人类中定位的同源物。纤连蛋白（FN1）的一个RFLP与α-抑制素（INHA）在5厘摩的距离处连锁。FN1基因座已被定位到绵羊2号染色体的2q41-q44区域，FN1与INHA之间的连锁将INHA也定位到绵羊的同一条染色体上。除了本文报道的新基因座外，该研究小组此前还发表了28个RFLP，这些与其他实验室发表的RFLP一起进行了整理。已报道绵羊中有86个基因座存在RFLP。54个基因座已被定位到16条不同的染色体上。