Bi Hongyan, Meng Sheng, Li Yan, Guo Kai, Chen Yupeng, Kong Jilie, Yang Pengyuan, Zhong Wei, Liu Baohong
Department of Chemistry, Fudan University, Shanghai 200433, People's Republic of China.
Lab Chip. 2006 Jun;6(6):769-75. doi: 10.1039/b600326e. Epub 2006 Mar 31.
A protein-resistant surface has been constructed on the poly(methyl methacrylate) (PMMA) microfluidic chips based on a one-step modification. The copolymer of butyl methacrylate (BMA) and poly(ethylene glycol) methyl ether methacrylate (PEGMA) is synthesized to introduce a dense PEG molecular brush-like coating on the PMMA microchannel surfaces via the anchoring effect of the hydrophobic BMA units. The PEGMA segments could produce hydrophilic domains formed on the interface so as to achieve stable electroosmotic flow, and less nonspecific adsorption toward biomolecules. The modification procedure and the properties of the poly(BMA-co-PEGMA)-coated surface have been characterized by FT-IR spectroscopy, confocal fluorescence microscopy, X-ray photoelectron spectroscopy and scanning electron microscopy. The water contact angle and electroosmotic flow of PEG-modified PMMA microchip are measured to be 36 degrees and 5.4 x 10(-4) cm(2) V(-1) s(-1), while those of 73 degrees and 1.9 x 10(-4) cm(2) V(-1) s(-1) for native one, respectively. The PEG-modified microchip has been applied for the electrophoresis separation of proteins, corresponding to the theoretical efficiencies about 16 300 and 412 300 plates m(-1). In the interest of achieving efficient separation while minimizing biofoulings from the serum and plasma, the fabrication of PEG-coated microfluidic chips would provide a biocompatible platform for complex biological analysis.
通过一步修饰法在聚甲基丙烯酸甲酯（PMMA）微流控芯片上构建了一种抗蛋白质表面。合成了甲基丙烯酸丁酯（BMA）和聚乙二醇甲基醚甲基丙烯酸酯（PEGMA）的共聚物，通过疏水性BMA单元的锚固作用，在PMMA微通道表面引入致密的PEG分子刷状涂层。PEGMA链段可在界面上形成亲水域，从而实现稳定的电渗流，并减少对生物分子的非特异性吸附。通过傅里叶变换红外光谱、共聚焦荧光显微镜、X射线光电子能谱和扫描电子显微镜对聚（BMA-co-PEGMA）涂层表面的修饰过程和性能进行了表征。PEG修饰的PMMA微芯片的水接触角和电渗流分别测得为36度和5.4×10⁻⁴ cm² V⁻¹ s⁻¹，而未修饰的微芯片分别为73度和1.9×10⁻⁴ cm² V⁻¹ s⁻¹。PEG修饰的微芯片已应用于蛋白质的电泳分离，理论塔板数分别约为16300和412300块/m。为了在尽量减少血清和血浆生物污染的同时实现高效分离，PEG涂层微流控芯片的制造将为复杂生物分析提供一个生物相容性平台。
