Jung Jaeyeon, Na Kyunga, Shin Byungcheol, Kim Okgene, Lee Jonghwan, Yun Kyusik, Hyun Jinho
Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, South Korea.
J Biomater Sci Polym Ed. 2008;19(2):161-73. doi: 10.1163/156856208783432507.
This paper investigates the chemical modification of a cell-repellent poly(ethylene glycol) (PEG)-based polymer to enhance its hydrophilicity with sulfonate groups, and its application in the fabrication of a cell microarray. First, a polymer comprised of a methyl methacrylate (MMA) backbone with PEG side-chains (PMMA-b-PEG) was synthesized from three monomers by radical polymerization and purified. Despite the hydrophilic side-groups in the amphiphilic polymer, the backbone structure's hydrophobicity allows for local adsorption of biomolecules in incubation media with or without serum. To enhance the hydrophilicity of the polymer, we tethered sulfonate groups to the hydroxyl groups on the PEG side chains (PMMA-b-PEG-SO3). The sulfate groups' physical and mechanical movement competitively repels biomolecules approaching the PMMA-b-PEG surface. Polymers modified with sulfonate were characterized by contact angle measurement, FT-IR, NMR, AFM and GPC. PMMA-b-PEG and PMMA-b-PEG-SO3 were successfully micropatterned on polystyrene and glass surfaces, and cell attachment was performed in either serum-free or serum-containing media, resulting in highly resolved cell micropatterns.
本文研究了一种基于聚乙二醇（PEG）的细胞排斥聚合物的化学改性，以通过磺酸基团增强其亲水性，并研究其在细胞微阵列制备中的应用。首先，通过自由基聚合由三种单体合成了一种由带有PEG侧链的甲基丙烯酸甲酯（MMA）主链组成的聚合物（PMMA-b-PEG）并进行了纯化。尽管两亲聚合物中存在亲水性侧基，但主链结构的疏水性使得生物分子在有血清或无血清的孵育介质中能够局部吸附。为了提高聚合物的亲水性，我们将磺酸基团连接到PEG侧链上的羟基上（PMMA-b-PEG-SO3）。硫酸基团的物理和机械运动竞争性地排斥接近PMMA-b-PEG表面的生物分子。用磺酸改性的聚合物通过接触角测量、傅里叶变换红外光谱（FT-IR）、核磁共振（NMR）、原子力显微镜（AFM）和凝胶渗透色谱（GPC）进行表征。PMMA-b-PEG和PMMA-b-PEG-SO3成功地在聚苯乙烯和玻璃表面进行了微图案化，并且在无血清或含血清的培养基中进行细胞附着，从而得到高度分辨的细胞微图案。
