Rice Olivia, Surian Allison, Chen Yupeng
Department of Biomedical Engineering, University of Connecticut, Storrs, CT, USA.
J Tissue Eng. 2022 May 14;13:20417314221095997. doi: 10.1177/20417314221095997. eCollection 2022 Jan-Dec.
The blood-brain barrier (BBB) is the most specialized biological barrier in the body. This configuration of specialized cells protects the brain from invasion of molecules and particles through formation of tight junctions. To learn more about transport to the brain, in vitro modeling of the BBB is continuously advanced. The types of models and cells selected vary with the goal of each individual study, but the same validation methods, quantification of tight junctions, and permeability assays are often used. With Transwells and microfluidic devices, more information regarding formation of the BBB has been observed. Disease models have been developed to examine the effects on BBB integrity. The goal of modeling is not only to understand normal BBB physiology, but also to create treatments for diseases. This review will highlight several recent studies to show the diversity in model selection and the many applications of BBB models in in vitro research.
血脑屏障（BBB）是人体最特殊的生物屏障。这种特殊细胞的结构通过形成紧密连接来保护大脑免受分子和颗粒的侵入。为了更多地了解物质向大脑的转运，血脑屏障的体外建模技术不断进步。所选择的模型和细胞类型因每项研究的目标而异，但通常会使用相同的验证方法、紧密连接的量化以及通透性测定。借助Transwell和微流控装置，人们观察到了更多关于血脑屏障形成的信息。已经开发出疾病模型来研究对血脑屏障完整性的影响。建模的目标不仅是了解正常的血脑屏障生理学，还在于开发疾病治疗方法。本综述将重点介绍几项近期研究，以展示模型选择的多样性以及血脑屏障模型在体外研究中的众多应用。
