Hahn A
Radiat Environ Biophys. 1975;11(4):265-9. doi: 10.1007/BF01326748.
The radioprotective effect of WR 2721 on catalase and the type and loci of its interaction with the enzyme have been investigated by means of spectrophotometric and electron spin resonance, (ESR) methods. The radiation damage, indicated by a change in enzymatic activity and in the Soret absorption band, has been the less the larger the WR 2721 concentration. In the case of ESR investigations, addition of WR 2721 has resulted in a reduction of the spin concentration of Cu-2+. Since cysteamine has exhibited similar results, however, to a lesser extent, it can be assumed that the RS-ions are responsible for the protective effect. From the results obtained it can be concluded that (the dephosphorilized) WR 2721 forms a complex with the enzyme and acts as an electron donor.
已通过分光光度法和电子自旋共振（ESR）方法研究了WR 2721对过氧化氢酶的辐射防护作用及其与该酶相互作用的类型和位点。酶活性和Soret吸收带的变化表明，辐射损伤程度随WR 2721浓度的增加而减小。在ESR研究中，添加WR 2721导致Cu-2+的自旋浓度降低。然而，由于半胱胺也显示出类似结果，但程度较小，因此可以认为RS-离子是产生保护作用的原因。从所得结果可以得出结论，（去磷酸化的）WR 2721与该酶形成复合物并作为电子供体起作用。
