Shumyantseva Victoria V, Bulko Tatiana V, Suprun Elena V, Chalenko Yaroslava M, Vagin Michail Yu, Rudakov Yurii O, Shatskaya Marina A, Archakov Alexander I
Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya St. 10, Moscow 119121, Russia.
Biochim Biophys Acta. 2011 Jan;1814(1):94-101. doi: 10.1016/j.bbapap.2010.07.008. Epub 2010 Jul 25.
In this paper we summarized our experimental data on the electrochemical reduction of cytochrome P450. Electrode/cytochrome P450 systems were analyzed in terms of the mechanisms underlying P450-catalyzed reactions. Bioelectrocatalysis-based screening of potential substrates or inhibitors of cytochrome P450, stoichiometry of the electrocatalytic cycle, redox thermodynamics and the peroxide shunt pathway were described. Characteristics, performance and potential application of cytochrome P450-electrodes are discussed.
在本文中，我们总结了关于细胞色素P450电化学还原的实验数据。根据P450催化反应的潜在机制，对电极/细胞色素P450系统进行了分析。描述了基于生物电催化的细胞色素P450潜在底物或抑制剂的筛选、电催化循环的化学计量、氧化还原热力学以及过氧化物分流途径。讨论了细胞色素P450电极的特性、性能和潜在应用。
