Puel Caroline, Mathey Jacinthe, Agalias Apostolis, Kati-Coulibaly Séraphin, Mardon Julie, Obled Christiane, Davicco Marie-Jeanne, Lebecque Patrice, Horcajada Marie-Noelle, Skaltsounis Alexios L, Coxam Véronique
Unité des Maladies Métaboliques et Micronutriments, INRA Theix, 63122 Saint Genès-Champanelle, France.
Clin Nutr. 2006 Oct;25(5):859-68. doi: 10.1016/j.clnu.2006.03.009. Epub 2006 Jun 5.
BACKGROUND & AIMS: This study was carried out to assess the dose-dependent bone-sparing effect of oleuropein, an olive oil phenolic compound with anti-inflammatory and anti-oxidative properties, on bone loss induced by talc granulomatosis in oestrogen-deficient rat.
Among 98 rats, 20 were sham-operated (SH) while the others (78) were ovariectomised (OVX). The SH and 26 OVX rats (controls) were given a standard diet for 100 days. The 52 remaining OVX rats were allocated to 4 groups that received oleuropein at 2.5, 5, 10 or 15 mg/kg body weight per day for 100 days. Three weeks before necropsy, an inflammation was induced by subcutaneous injections of talc in half of the SH and OVX rats and in all oleuropein-treated animals.
Castration was associated with a decreased bone mineral density (BMD). In OVX rats, inflammation, characterised by an increase of the spleen weight and plasma fibrinogen levels, exacerbated this bone loss, as shown by values of BMD of the total femur metaphyseal and diaphyseal subregions. The 4 doses of oleuropein reduced bone loss and improved inflammatory biomarkers excepted for 5mg/kg BW.
Every dose of oleuropein elicited protective effects on bone mass in this model of ovariectomy associated with inflammation, probably by modulating inflammatory parameters.
本研究旨在评估橄榄苦苷（一种具有抗炎和抗氧化特性的橄榄油酚类化合物）对雌激素缺乏大鼠滑石肉芽肿病所致骨质流失的剂量依赖性保骨作用。
98只大鼠中，20只进行假手术（SH），其余78只进行卵巢切除术（OVX）。SH组和26只OVX大鼠（对照组）给予标准饮食100天。其余52只OVX大鼠分为4组，分别给予2.5、5、10或15mg/kg体重的橄榄苦苷，持续100天。在尸检前3周，对一半的SH和OVX大鼠以及所有接受橄榄苦苷治疗的动物皮下注射滑石粉诱导炎症。
去势与骨矿物质密度（BMD）降低有关。在OVX大鼠中，以脾脏重量和血浆纤维蛋白原水平增加为特征的炎症加剧了这种骨质流失，总股骨干骺端和骨干亚区域的BMD值表明了这一点。除5mg/kg体重剂量外，4种剂量的橄榄苦苷均减少了骨质流失并改善了炎症生物标志物。
在这种与炎症相关的卵巢切除模型中，每剂量的橄榄苦苷都对骨量产生保护作用，可能是通过调节炎症参数实现的。
