Puel Caroline, Mardon Julie, Kati-Coulibaly Séraphin, Davicco Marie-Jeanne, Lebecque Patrice, Obled Christiane, Rock Edmond, Horcajada Marie-Noelle, Agalias Apostolos, Skaltsounis Leandros A, Coxam Véronique
Unité des Maladies Métaboliques et Micronutriments, INRA Theix, 63122 Saint Genes-Champanelle, France.
Br J Nutr. 2007 May;97(5):1012-20. doi: 10.1017/S0007114507659030.
This study was conducted to determine whether olive fruits, rich in micronutrients, might improve bone loss in ovariectomized (OVX) rats (an experimental model of postmenopausal osteoporosis) and in OVX rats with granulomatosis inflammation (a model of senile osteoporosis). Six-month-old Wistar female rats underwent ovariectomy and were then immediately treated orally by substituting oil in the diet by 10 g/d green Lucques olives or 6 g/d black Lucques olives for each rat for 84 days. OVX rats and sham-operated controls received the same diet with oil. Three weeks before the end of the experiment, subcutaneous inflammation was provoked by injections of sterile magnesium silicate in half the animals in each group. In OVX rats, granulomatosis inflammation, characterized by a rise in inflammatory parameters such as fibrinogen, alpha1-acid glycoprotein, spleen weight and granulocyte level, and an impairment of oxidative status (as shown by a decrease in plasma antioxidant capacity, a higher rate of isoprostane excretion) elicited a bone loss in the whole femur and in the metaphyseal areas considered on their own. Whereas green olives had no effect on osteopenia, consumption of the black variety prevented bone loss in the whole femur and at cortical sites in those oestrogen-deficient animals with talc inflammation (diaphyseal bone mineral density: black olives and inflammation 0-2323 (SE 0.0026) v. ovariectomy and inflammation 0.2117 (SE 0.0030); P=0.027). This bone-sparing effect seemed to result from an improvement in the inflammatory and oxidative status. The present data show that black olives are able to prevent bone loss in an experimental model of senile osteoporosis (oestrogen-deficient rats in which a low-grade inflammation was induced by talc injection).
本研究旨在确定富含微量营养素的橄榄果实是否能改善去卵巢（OVX）大鼠（绝经后骨质疏松症的实验模型）以及患有肉芽肿性炎症的OVX大鼠（老年性骨质疏松症模型）的骨质流失。6月龄的Wistar雌性大鼠接受去卵巢手术，然后立即通过用10克/天的绿色吕克橄榄或6克/天的黑色吕克橄榄替代饮食中的油进行口服治疗，持续84天。OVX大鼠和假手术对照组接受相同的含油饮食。在实验结束前3周，每组一半的动物通过注射无菌硅酸镁引发皮下炎症。在OVX大鼠中，肉芽肿性炎症表现为纤维蛋白原、α1-酸性糖蛋白、脾脏重量和粒细胞水平等炎症参数升高，以及氧化状态受损（如血浆抗氧化能力下降、异前列腺素排泄率升高所示），导致整个股骨和单独考虑的干骺端区域骨质流失。绿色橄榄对骨质减少没有影响，而食用黑色橄榄可预防滑石粉炎症的雌激素缺乏动物整个股骨和皮质部位的骨质流失（骨干骨矿物质密度：黑色橄榄和炎症组为0.2323（标准误0.0026），而去卵巢和炎症组为0.2117（标准误0.0030）；P = 0.027）。这种骨质保护作用似乎源于炎症和氧化状态的改善。目前的数据表明，黑色橄榄能够预防老年性骨质疏松症实验模型（通过注射滑石粉诱导低度炎症的雌激素缺乏大鼠）中的骨质流失。
