人血小板中的外肽酶：生物活性肽蛋白水解调节的一个指征

Exopeptidases in human platelets: an indication for proteolytic modulation of biologically active peptides.

作者信息

Scharpé S L, Vanhoof G C, De Meester I A, Hendriks D F, van Sande M E, Muylle L M, Yaron A

机构信息

Department of Clinical Biochemistry, University of Antwerp, Belgium.

出版信息

Clin Chim Acta. 1991 Jan 15;195(3):125-31. doi: 10.1016/0009-8981(91)90132-v.

DOI:10.1016/0009-8981(91)90132-v

PMID:1674236

Abstract

The determination in human platelets of four exopeptidases--aminopeptidase P, dipeptidyl peptidase IV, carboxypeptidase N, and angiotensin converting enzyme--by means of fluorometric or liquid chromatography techniques was carried out. The results obtained show that the specific activities of dipeptidyl peptidase IV, carboxypeptidase N, and angiotensin converting enzyme in intact and disrupted platelets are small compared to their specific activities in serum. However, for aminopeptidase P the specific activity of this enzyme is much higher in platelets than in serum. This suggests that circulating platelets may have a significant role as scavengers for circulating peptides containing bonds susceptible for aminopeptidase P.

摘要

采用荧光法或液相色谱技术对人血小板中的四种外肽酶——氨肽酶P、二肽基肽酶IV、羧肽酶N和血管紧张素转换酶进行了测定。所得结果表明，完整血小板和破碎血小板中二肽基肽酶IV、羧肽酶N和血管紧张素转换酶的比活性与其在血清中的比活性相比很低。然而，对于氨肽酶P，该酶在血小板中的比活性比在血清中高得多。这表明循环血小板可能作为含有易被氨肽酶P作用的肽键的循环肽的清除剂发挥重要作用。