环己亚胺对大鼠肝蛋白质生物合成的影响。
Effect of cycloheximide on protein biosynthesis in rat liver.
机构信息
Department of Biochemistry, University of Cambridge.
出版信息
Biochem J. 1966 Dec;101(3):627-31. doi: 10.1042/bj1010627.
Abstract
- The liver ribosomes of rats given cycloheximide by intraperitoneal injection incorporate less amino acid into protein than ribosomes from control rat liver when they are incubated in vitro with excess of Sephadex-treated cell sap. The effect is rapid, marked and persistent. 2. Cell sap from liver of cycloheximide-treated animals is inhibitory but the inhibition can be relieved almost entirely by treating the cell sap with Sephadex. No damage has been done to the cell-sap factors: it is suggested that the dissolved cycloheximide in the cell sap causes the inhibition. 3. Cycloheximide added in vitro inhibits amino acid incorporation into protein in the presence or absence of polyuridylic acid. The inhibition is lessened by addition of excess of cell sap but is not abolished. 4. The differences between these results and those obtained with mouse liver (Trakatellis, Montjar & Axelrod, 1965) might arise because of species differences in sensitivity to the drug.
摘要
给大鼠腹腔内注射环己亚胺后，其肝脏核糖体与对照大鼠肝脏核糖体相比，在与过量的经葡聚糖处理的胞浆提取物体外孵育时，掺入蛋白质的氨基酸较少。这种效应迅速、显著且持久。
环己亚胺处理动物的肝胞浆提取物具有抑制作用，但用葡聚糖处理该胞浆提取物几乎可完全缓解抑制。胞浆因子未受损伤：据认为，胞浆中溶解的环己亚胺引起了抑制。
环己亚胺在体外加入时，无论是否存在多聚尿嘧啶核苷酸，均会抑制氨基酸掺入蛋白质。添加过量的胞浆提取物可减轻抑制作用，但不能消除抑制作用。
这些结果与用鼠肝（Trakatellis、Montjar 和 Axelrod，1965）获得的结果之间的差异可能是由于对药物的敏感性在物种间存在差异。
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