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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1966 Sep;100(3):815-22. doi: 10.1042/bj1000815.

Phytohaemagglutinin stimulates the transformation into blast cells of human lymphocytes incubated in vitro. This transformation is accompanied by an increase in the incorporation of [(14)C]leucine into protein and [(3)H]uridine into RNA. 2. The incorporation of [(14)C]leucine by cultures grown in the presence or absence of phytohaemagglutinin is inhibited to the same extent by cycloheximide, a known inhibitor of protein synthesis. 3. Lymphocytes grown without phytohaemagglutin synthesize mainly non-ribosomal RNA. [(3)H]Uridine incorporation by these cells was increased by cycloheximide. 4. Lymphocytes incubated with phytohaemagglutinin begin to synthesize substantial quantities of ribosomal RNA. Under these conditions [(3)H]uridine incorporation was partially inhibited by cycloheximide. This inhibition is shown to be largely a result of inhibition of the synthesis of ribosomal RNA.

植物血凝素可刺激体外培养的人淋巴细胞转化为母细胞。这种转化伴随着[（14）C]亮氨酸掺入蛋白质以及[（3）H]尿苷掺入RNA的增加。2. 已知的蛋白质合成抑制剂环己酰亚胺对在有或没有植物血凝素存在的情况下培养的细胞中[（14）C]亮氨酸的掺入抑制程度相同。3. 在没有植物血凝素的情况下生长的淋巴细胞主要合成非核糖体RNA。这些细胞中[（3）H]尿苷的掺入量因环己酰亚胺而增加。4. 用植物血凝素孵育的淋巴细胞开始合成大量核糖体RNA。在这些条件下，[（3）H]尿苷的掺入被环己酰亚胺部分抑制。这种抑制作用在很大程度上是由于核糖体RNA合成受到抑制所致。

Biochem J. 1966 Sep;100(3):815-22. doi: 10.1042/bj1000815.

Phytohaemagglutinin stimulates the transformation into blast cells of human lymphocytes incubated in vitro. This transformation is accompanied by an increase in the incorporation of [(14)C]leucine into protein and [(3)H]uridine into RNA. 2. The incorporation of [(14)C]leucine by cultures grown in the presence or absence of phytohaemagglutinin is inhibited to the same extent by cycloheximide, a known inhibitor of protein synthesis. 3. Lymphocytes grown without phytohaemagglutin synthesize mainly non-ribosomal RNA. [(3)H]Uridine incorporation by these cells was increased by cycloheximide. 4. Lymphocytes incubated with phytohaemagglutinin begin to synthesize substantial quantities of ribosomal RNA. Under these conditions [(3)H]uridine incorporation was partially inhibited by cycloheximide. This inhibition is shown to be largely a result of inhibition of the synthesis of ribosomal RNA.

植物血凝素可刺激体外培养的人淋巴细胞转化为母细胞。这种转化伴随着[（14）C]亮氨酸掺入蛋白质以及[（3）H]尿苷掺入RNA的增加。2. 已知的蛋白质合成抑制剂环己酰亚胺对在有或没有植物血凝素存在的情况下培养的细胞中[（14）C]亮氨酸的掺入抑制程度相同。3. 在没有植物血凝素的情况下生长的淋巴细胞主要合成非核糖体RNA。这些细胞中[（3）H]尿苷的掺入量因环己酰亚胺而增加。4. 用植物血凝素孵育的淋巴细胞开始合成大量核糖体RNA。在这些条件下，[（3）H]尿苷的掺入被环己酰亚胺部分抑制。这种抑制作用在很大程度上是由于核糖体RNA合成受到抑制所致。