从金黄色葡萄球菌中释放表面定位青霉素酶。

Growth of Staphylococcus aureus (8325; alphai(-)p(+)), constitutive for the production of penicillinase, in CY medium results in about 40% of the enzyme being free in the medium. By modifying the medium, 98% of the enzyme remains cell-bound. 2. Part of this is bound ionically to the surface of the cell wall and may be liberated instantaneously by certain inorganic anions. Maximum liberation was achieved with either phosphate or arsenate, both of which showed marked pH-dependence. 3. Polyanions that do not penetrate the cell wall, such as heparin, RNA and dextran sulphate, are also effective in liberating penicillinase. 4. Polyanions added to the growth medium prevent the appearance of ionically bound penicillinase owing to their strong affinity for the sites on the cell wall required for binding of the enzyme.

产青霉素酶的金黄色葡萄球菌（8325；alpha(-) p(+)）在 CY 培养基中生长，约有 40%的酶游离在培养基中。通过对培养基进行改良，98%的酶仍与细胞结合。
其中一部分通过离子键与细胞壁表面结合，某些无机阴离子可立即将其释放出来。用磷酸盐或砷酸盐可实现最大程度的释放，这两种物质均表现出明显的 pH 依赖性。
不穿透细胞壁的聚阴离子，如肝素、RNA 和硫酸右旋糖苷，也能有效地释放青霉素酶。
添加到生长培养基中的聚阴离子由于与细胞壁上结合酶所需的位点具有很强的亲和力，因此会阻止离子结合的青霉素酶的出现。

Growth of Staphylococcus aureus (8325; alphai(-)p(+)), constitutive for the production of penicillinase, in CY medium results in about 40% of the enzyme being free in the medium. By modifying the medium, 98% of the enzyme remains cell-bound. 2. Part of this is bound ionically to the surface of the cell wall and may be liberated instantaneously by certain inorganic anions. Maximum liberation was achieved with either phosphate or arsenate, both of which showed marked pH-dependence. 3. Polyanions that do not penetrate the cell wall, such as heparin, RNA and dextran sulphate, are also effective in liberating penicillinase. 4. Polyanions added to the growth medium prevent the appearance of ionically bound penicillinase owing to their strong affinity for the sites on the cell wall required for binding of the enzyme.

产青霉素酶的金黄色葡萄球菌（8325；alpha(-) p(+)）在 CY 培养基中生长，约有 40%的酶游离在培养基中。通过对培养基进行改良，98%的酶仍与细胞结合。
其中一部分通过离子键与细胞壁表面结合，某些无机阴离子可立即将其释放出来。用磷酸盐或砷酸盐可实现最大程度的释放，这两种物质均表现出明显的 pH 依赖性。
不穿透细胞壁的聚阴离子，如肝素、RNA 和硫酸右旋糖苷，也能有效地释放青霉素酶。
添加到生长培养基中的聚阴离子由于与细胞壁上结合酶所需的位点具有很强的亲和力，因此会阻止离子结合的青霉素酶的出现。

Liberation of surface-located penicillinase from Staphylococcus aureus.