Greenway D L, Dyke K G
J Gen Microbiol. 1983 Aug;129(8):2457-65. doi: 10.1099/00221287-129-8-2457.
The presence of a plasmid conferring resistance to penicillin (PC plasmid, e.g. pI258blaI-) in Staphylococcus aureus NCTC 8325 increases the sensitivity of such a bacterium to the growth inhibitory effects of linoleic acid, whereas a plasmid conferring resistance to tetracycline does not affect linoleic acid sensitivity. The increased linoleic acid sensitivity of bacteria containing a PC plasmid may be related to the penicillinase protein itself since (i) strains having inducible penicillinase show increased sensitivity only after induction, (ii) strains in which penicillinase is directed from chromosomal or plasmid-borne genes show similar increased linoleic acid sensitivity and (iii) notwithstanding the above, the linoleic acid inhibitory effect is enhanced in a strain in which penicillinase activity is greatly reduced by a point mutation in the structural gene for penicillinase. The enhanced linoleic acid sensitivity seems to require the membrane-bound penicillinase since added extracellular penicillinase does not confer this sensitivity, and there appears to be a specific interaction between the membrane-bound penicillinase activity and linoleic acid.
在金黄色葡萄球菌NCTC 8325中，携带对青霉素具有抗性的质粒（PC质粒，例如pI258blaI-）会增加该细菌对亚油酸生长抑制作用的敏感性，而携带对四环素具有抗性的质粒则不会影响亚油酸敏感性。含有PC质粒的细菌对亚油酸敏感性增加可能与青霉素酶蛋白本身有关，因为：（i）具有可诱导青霉素酶的菌株仅在诱导后才表现出增加的敏感性；（ii）青霉素酶由染色体或质粒携带基因指导表达的菌株表现出类似的亚油酸敏感性增加；（iii）尽管如此，在一个由于青霉素酶结构基因中的点突变而使青霉素酶活性大大降低的菌株中，亚油酸的抑制作用仍会增强。增强的亚油酸敏感性似乎需要膜结合青霉素酶，因为添加的细胞外青霉素酶不会赋予这种敏感性，并且膜结合青霉素酶活性与亚油酸之间似乎存在特异性相互作用。
