Coles N W, Gross R
Antimicrob Agents Chemother. 1973 Aug;4(2):178-85. doi: 10.1128/AAC.4.2.178.
Conditions suitable for induced formation and secretion of penicillinase (EC 3.5.2.6) in protoplasts of Staphylococcus aureus were determined. No requirement was found for cells to be exposed to inducer prior to formation of protoplasts. Neither cell wall components nor mesosomes appeared to be necessary for induction or secretion. In medium containing 1.1 M sucrose about half of the formed enzyme was soluble, whereas in medium containing 0.37 M sodium succinate only about 10% of the penicillinase remained protoplast-bound. Low concentrations of polyanions (dextran sulfate and potassium polyvinyl-sulfonate) inhibited the formation of induced penicillinase, as did 4-acetamido-4'-isothiocyano-stilbene 2,2'-disulfonic acid. None of these compounds inhibited the activity of native penicillinase, and none would be expected to pass through the protoplast membrane. Penicillinase, denatured in 4 M urea, could be renatured by dilution in the presence of benzylpenicillin, and the above three inhibitors interfered strongly with this process. The results are taken as evidence that penicillinase may be secreted through the protoplast membrane in an incompletely folded form.
确定了适合在金黄色葡萄球菌原生质体中诱导形成和分泌青霉素酶（EC 3.5.2.6）的条件。未发现细胞在形成原生质体之前需要暴露于诱导剂。细胞壁成分和中介体似乎都不是诱导或分泌所必需的。在含有1.1 M蔗糖的培养基中，约一半形成的酶是可溶的，而在含有0.37 M琥珀酸钠的培养基中，只有约10%的青霉素酶仍与原生质体结合。低浓度的聚阴离子（硫酸葡聚糖和聚乙烯磺酸钾）抑制诱导型青霉素酶的形成，4-乙酰氨基-4'-异硫氰基芪2,2'-二磺酸也是如此。这些化合物均未抑制天然青霉素酶的活性，且预计它们都不会穿过原生质体膜。在4 M尿素中变性的青霉素酶，在苄青霉素存在下通过稀释可复性，上述三种抑制剂强烈干扰这一过程。这些结果被视为青霉素酶可能以未完全折叠的形式通过原生质体膜分泌的证据。
