病毒感染对仓鼠幼肾细胞中放线菌素抗性核糖核酸的稳定性和合成的影响。
Effect of virus infection on the stability and synthesis of actinomycin-resistant ribonucleic acid in baby-hamster kidney cells.
机构信息
The Animal Virus Research Institute, Pirbright, Surrey.
出版信息
Biochem J. 1967 Dec;105(3):987-93. doi: 10.1042/bj1050987.
Abstract
- The sucrose-gradient pattern of (32)P-labelled RNA synthesized in actinomycintreated baby-hamster kidney cells infected with foot-and-mouth-disease virus depends greatly on the period of labelling. 2. Fractions are formed in infected cells that sediment at 12-20s and have the same base composition as similar fractions found in non-infected cells that have been treated with actinomycin. 3. In the presence of guanidine, which completely inhibits viral RNA synthesis, these fractions are labelled to a greater extent than in non-infected cells.
摘要
在感染口蹄疫病毒的仓鼠肾细胞中，经放线菌素处理后，(32)P 标记的 RNA 合成的蔗糖梯度图谱在很大程度上取决于标记的时期。
在感染细胞中形成了在 12-20s 处沉降的级分，并且其碱基组成与用放线菌素处理的未感染细胞中的类似级分相同。
在胍存在的情况下，完全抑制病毒 RNA 合成，这些级分的标记程度比未感染细胞更高。
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