一项关于口蹄疫病毒诱导产生的异质性37S核糖核酸的研究。
A study of the heterogeneous 37S ribonucleic acid induced by foot-and-mouth-disease virus.
作者信息
Wild T F, Martin S J, Brown F
出版信息
Biochem J. 1968 Apr;107(3):395-401. doi: 10.1042/bj1070395.
Abstract
- The 37s RNA induced in baby-hamster kidney cells by infection with foot-and-mouth-disease virus was examined on sucrose gradients and by filtration through Sepharose 4B. 2. The RNA sedimented faster (37s) and as a broader band than the 35s RNA from purified virus. 3. Treatment with deoxyribonuclease, Pronase or amylase did not alter the sedimentation profile of the 37s RNA. 4. Treatment of individual fractions of the RNA with phenol, dimethyl sulphoxide or methylCellosolve did not decrease the sedimentation rate of the faster-sedimenting molecules. 5. Sedimentation in sucrose gradients of different ionic strengths or containing EDTA had no effect on the heterogeneous nature of the profile. 6. On filtration through Sepharose 4B columns, the 37s virus-induced RNA was eluted before viral RNA. 7. Only 20% of the rapidly sedimenting RNA was incorporated into complete virus particles.
摘要
- 用口蹄疫病毒感染幼仓鼠肾细胞后诱导产生的37s RNA，通过蔗糖梯度离心和经琼脂糖4B过滤进行检测。2. 该RNA沉降速度比纯化病毒的35s RNA更快（37s），且条带更宽。3. 用脱氧核糖核酸酶、链霉蛋白酶或淀粉酶处理不会改变37s RNA的沉降图谱。4. 用苯酚、二甲基亚砜或甲基溶纤剂处理RNA的各个组分，不会降低沉降速度较快的分子的沉降速率。5. 在不同离子强度或含有乙二胺四乙酸（EDTA）的蔗糖梯度中沉降，对图谱的不均一性没有影响。6. 经琼脂糖4B柱过滤时，37s病毒诱导的RNA比病毒RNA先洗脱出来。7. 快速沉降的RNA中只有20%被整合到完整的病毒颗粒中。
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