Poirier J, Hess M, May P C, Finch C E
McGill Center for Studies in Aging, Montreal General Hospital, Que., Canada.
Brain Res Mol Brain Res. 1991 Feb;9(3):191-5. doi: 10.1016/0169-328x(91)90002-f.
Evidence is given for altered gene expression in the hippocampus in response to entorhinal cortex lesioning. Three RNA markers encoding glial fibrillary acidic protein, apolipoprotein E and alpha-tubulin were isolated from a rat hippocampal cDNA library by differential screening with cDNA probes from entorhinal cortex lesioned and control rat hippocampus RNA. By Northern blot analysis, mRNA for apolipoprotein E and alpha-tubulin increased to peak around 6 days after the lesion and returned to near control level at 30 days. The increased synthesis of both mRNAs coincides with the acute phase of synaptogenesis, protein synthesis, and polyribosomes accumulation in the deafferented hippocampal area.
有证据表明，内嗅皮层损伤会导致海马体基因表达发生改变。通过用来自内嗅皮层损伤大鼠和对照大鼠海马体RNA的cDNA探针进行差异筛选，从大鼠海马体cDNA文库中分离出了三种编码胶质纤维酸性蛋白、载脂蛋白E和α-微管蛋白的RNA标记物。通过Northern印迹分析，载脂蛋白E和α-微管蛋白的mRNA在损伤后约6天增加至峰值，并在30天时恢复到接近对照水平。两种mRNA合成的增加与去传入海马区突触发生、蛋白质合成和多核糖体积累的急性期相吻合。
